Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 198117..198742 Replicon plasmid pCP301
Accession NC_004851
Organism Shigella flexneri 2a str. 301

Toxin (Protein)


Gene name vapC Uniprot ID O06662
Locus tag CP0245 Protein ID NP_858378.1
Coordinates 198117..198515 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID O06663
Locus tag CP0246 Protein ID NP_858379.1
Coordinates 198515..198742 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP0237 193647..194597 + 951 NP_858370.1 hypothetical protein -
CP0238 194662..195606 + 945 NP_858371.1 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase -
CP0239 195760..196716 - 957 NP_858372.1 IS630 orf -
CP0242 197582..197884 - 303 NP_858375.1 IS600 ORF1 -
CP0243 197923..198108 + 186 NP_858376.1 DNA transport protein -
CP0245 198117..198515 - 399 NP_858378.1 plasmid maintenance protein Toxin
CP0246 198515..198742 - 228 NP_858379.1 plasmid maintenance protein Antitoxin
CP0247 198803..199195 + 393 NP_858380.1 oriT nicking and unwinding protein -
CP0248 199216..200580 + 1365 NP_858381.1 oriT nicking and unwinding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA 1..221618 221618


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14817.08 Da        Isoelectric Point: 8.5232

>T20976 NP_858378.1 NC_004851:c198515-198117 [Shigella flexneri 2a str. 301]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Download         Length: 399 bp

>T20976 NC_004851:c198515-198117 [Shigella flexneri 2a str. 301]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAAAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAACTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8565.62 Da        Isoelectric Point: 4.7513

>AT20976 NP_858379.1 NC_004851:c198742-198515 [Shigella flexneri 2a str. 301]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSTDFMDNREQPGMQERESF

Download         Length: 228 bp

>AT20976 NC_004851:c198742-198515 [Shigella flexneri 2a str. 301]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCACCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAAGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3TND
PDB 5ECD
PDB 5ECY
PDB 5ECW
PDB 5ED0

Antitoxin

Source ID Structure
PDB 3TND
AlphaFold DB O06663

References