Detailed information of TA system TA020975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 180540..181065
• Replicon: plasmid pCP301
• Accession: NC_004851
• Organism: Shigella flexneri 2a str. 301

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: CP0225
• Protein ID: NP_858358.3
• Coordinates: 180760..181065 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q99Q95
• Locus tag: CP0224
• Protein ID: NP_858357.1
• Coordinates: 180540..180758 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP0214	176266..176499	+	234	NP_858347.1	IS100 ORF2	-
CP0215	176551..176841	-	291	NP_858348.1	hypothetical protein	-
CP0216	176810..177061	-	252	NP_858349.1	hypothetical protein	-
CP0217	177513..177797	+	285	NP_858350.1	hypothetical protein	-
CP0218	177797..178072	+	276	NP_858351.1	hypothetical protein	-
CP0219	178125..178304	+	180	NP_858352.1	hypothetical protein	-
CP0220	178298..178801	-	504	NP_858353.1	IS1 ORF2	-
CP0221	178876..179178	+	303	NP_858354.1	IS1 encoded protein	-
CP0222	179387..179605	+	219	NP_858355.1	hypothetical protein	-
CP0223	179624..180004	+	381	NP_858356.1	ISSfl4 ORF3	-
CP0224	180540..180758	+	219	NP_858357.1	plasmid maintenance protein CcdA	Antitoxin
CP0225	180760..181065	+	306	NP_858358.3	plasmid maintenance protein CcdB	Toxin
CP0226	181458..183095	+	1638	NP_858359.2	invasion plasmid antigen, secreted by the Mxi-Spa secretion machinery	-
CP0227	183824..184414	+	591	NP_858360.1	protein OspG	-
CP0228	184482..184661	-	180	NP_858361.1	plasmid mobilization protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA	1..221618	221618
	-	inside	IScluster/Tn	-	ospI / ipaH9.8 / ospG	167032..187890	20858

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11736.59 Da
• Isoelectric Point: 6.4674

>T20975 NP_858358.3 NC_004851:180760-181065 [Shigella flexneri 2a str. 301]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T20975 NC_004851:180760-181065 [Shigella flexneri 2a str. 301]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8333.36 Da
• Isoelectric Point: 6.2838

>AT20975 NP_858357.1 NC_004851:180540-180758 [Shigella flexneri 2a str. 301]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT20975 NC_004851:180540-180758 [Shigella flexneri 2a str. 301]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: Q99Q95