Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 177513..178072 Replicon plasmid pCP301
Accession NC_004851
Organism Shigella flexneri 2a str. 301

Toxin (Protein)


Gene name relE Uniprot ID A0A0H2USR3
Locus tag CP0218 Protein ID NP_858351.1
Coordinates 177797..178072 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A2S4MRI9
Locus tag CP0217 Protein ID NP_858350.1
Coordinates 177513..177797 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP0210 172967..173239 - 273 NP_858343.1 iso-IS1 ORF1 -
CP0211 173438..174112 + 675 NP_858344.1 ISSfl4 ORF1 -
CP0212 174109..174456 + 348 NP_858345.1 ISSfl4 ORF2 -
CP0213 174476..176077 + 1602 NP_858346.1 ISSfl4 ORF3 -
CP0214 176266..176499 + 234 NP_858347.1 IS100 ORF2 -
CP0215 176551..176841 - 291 NP_858348.1 hypothetical protein -
CP0216 176810..177061 - 252 NP_858349.1 hypothetical protein -
CP0217 177513..177797 + 285 NP_858350.1 hypothetical protein Antitoxin
CP0218 177797..178072 + 276 NP_858351.1 hypothetical protein Toxin
CP0219 178125..178304 + 180 NP_858352.1 hypothetical protein -
CP0220 178298..178801 - 504 NP_858353.1 IS1 ORF2 -
CP0221 178876..179178 + 303 NP_858354.1 IS1 encoded protein -
CP0222 179387..179605 + 219 NP_858355.1 hypothetical protein -
CP0223 179624..180004 + 381 NP_858356.1 ISSfl4 ORF3 -
CP0224 180540..180758 + 219 NP_858357.1 plasmid maintenance protein CcdA -
CP0225 180760..181065 + 306 NP_858358.3 plasmid maintenance protein CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA 1..221618 221618
- inside IScluster/Tn - ospI / ipaH9.8 / ospG 167032..187890 20858


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10766.48 Da        Isoelectric Point: 9.9324

>T20974 NP_858351.1 NC_004851:177797-178072 [Shigella flexneri 2a str. 301]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T20974 NC_004851:177797-178072 [Shigella flexneri 2a str. 301]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10529.97 Da        Isoelectric Point: 6.2150

>AT20974 NP_858350.1 NC_004851:177513-177797 [Shigella flexneri 2a str. 301]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSW
ADSLSTDHLLPVPR

Download         Length: 285 bp

>AT20974 NC_004851:177513-177797 [Shigella flexneri 2a str. 301]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGTCCTGG
GCGGACAGCCTCAGTACTGACCATCTGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2USR3

Antitoxin

Source ID Structure
AlphaFold DB A0A2S4MRI9

References