Detailed information of TA system TA209736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1588972..1590667
• Replicon: chromosome
• Accession: NZ_CP079712
• Organism: Pseudomonas aeruginosa strain PAO1-UW

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: KXE08_RS07410
• Protein ID: WP_003112901.1
• Coordinates: 1588972..1589841 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: KXE08_RS07415
• Protein ID: WP_003112902.1
• Coordinates: 1589834..1590667 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXE08_RS07385 (KXE08_07370)	1584980..1585831	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
KXE08_RS07390 (KXE08_07375)	1585872..1586879	+	1008	WP_003112897.1	ABC transporter substrate-binding protein	-
KXE08_RS07395 (KXE08_07380)	1586876..1587652	+	777	WP_003112898.1	ABC transporter permease	-
KXE08_RS07400 (KXE08_07385)	1587658..1588419	+	762	WP_003112899.1	SDR family oxidoreductase	-
KXE08_RS07405 (KXE08_07390)	1588433..1588963	+	531	WP_003112900.1	cupin domain-containing protein	-
KXE08_RS07410 (KXE08_07395)	1588972..1589841	+	870	WP_003112901.1	alpha/beta hydrolase	Toxin
KXE08_RS07415 (KXE08_07400)	1589834..1590667	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
KXE08_RS07420 (KXE08_07405)	1590654..1591451	+	798	WP_003112903.1	SDR family oxidoreductase	-
KXE08_RS07425 (KXE08_07410)	1591444..1593126	+	1683	WP_003112904.1	thiamine pyrophosphate-binding protein	-
KXE08_RS07430 (KXE08_07415)	1593137..1593940	+	804	WP_003112905.1	aspartate dehydrogenase	-
KXE08_RS07435 (KXE08_07420)	1593952..1595436	+	1485	WP_003112906.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T209736 WP_003112901.1 NZ_CP079712:1588972-1589841 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T209736 NZ_CP104288:c2827255-2827079 [Stenotrophomonas maltophilia]
ATGAAGAGCAGGGACCTCATCCGGGATCTGGAAGCGGCATGTTGGATCTGTCGTCGGATCCGCGGATCCCATCATGTATT
CGTGCACCCGCAGCACCCTCACATCATCACCGTTCCTCAGCCAAGGAAGGATCTGGGGAAGGGCTGGTACAGGCGTTGCG
GAAGCTCGCAGGACTGA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT209736 WP_003112902.1 NZ_CP079712:1589834-1590667 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT209736 NZ_CP104288:c2827065-2826964 [Stenotrophomonas maltophilia]
ATGCGATATCCAGTCTTGATCGAGGCAGGCGATGAGCGCACCGCGTGGGGTGTGGTCGTTCCAGATCTGCCGGGGGTGCT
TCTCGGCGGCCGACACGCTTGA

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1