Detailed information of TA system TA209734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 36980..37505
• Replicon: plasmid pS468MPCH_P2
• Accession: NZ_CP079694
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC31_S468MPCH

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: KW564_RS28085
• Protein ID: WP_001159868.1
• Coordinates: 37200..37505 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: KW564_RS28080
• Protein ID: WP_000813634.1
• Coordinates: 36980..37198 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW564_RS28060 (KW564_28035)	32918..34195	-	1278	WP_016338369.1	HlyD family secretion protein	-
KW564_RS28520	34854..35390	+	537	WP_223825639.1	hypothetical protein	-
KW564_RS28525	35408..35731	+	324	WP_085842301.1	hypothetical protein	-
KW564_RS28070 (KW564_28045)	35772..36188	-	417	WP_048983057.1	type II toxin-antitoxin system VapC family toxin	-
KW564_RS28075 (KW564_28050)	36185..36415	-	231	WP_016338373.1	type II toxin-antitoxin system VapB family antitoxin	-
KW564_RS28080 (KW564_28055)	36980..37198	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KW564_RS28085 (KW564_28060)	37200..37505	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
KW564_RS28090 (KW564_28065)	37506..38312	+	807	WP_000016982.1	site-specific integrase	-
KW564_RS28095 (KW564_28070)	39086..39841	+	756	WP_000852146.1	replication initiation protein RepE	-
KW564_RS28100 (KW564_28075)	40429..41595	+	1167	WP_000772446.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrB1 / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaTEM-1B / tet(D)	-	1..55146	55146

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T209734 WP_001159868.1 NZ_CP079694:37200-37505 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T209734 NZ_CP104288:270307-270612 [Stenotrophomonas maltophilia]
ATGAAGATCCAGCGTACCCGTCAGTTCGCGACATGGATTGACGCTCTCAAGGACGTAACCGCACGCGCTCGCATCCTGGC
CCGTATCGGTCGGCTCGCTGAAGGCCATCCTGGTGATCACCGCTATCTGGCGGATGGCGTTTCTGAATTGCGTATCGATG
CAGGACCCGGTTACCGCGTGTACTACACCCAGCGGGGCAGGCAGTTGGTGATTCTTCTGGTCGGAGGCGACAAGGGTTCG
CAACAGCGCGATATAGAGAAGGCCAAGGAGATTGCGCGTGCCCTGCGAGCATCGAACTGGTTGTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT209734 WP_000813634.1 NZ_CP079694:36980-37198 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT209734 NZ_CP104288:270653-270955 [Stenotrophomonas maltophilia]
ATGGCTATCAAGAAGAAAGTTGAACTCGAAGCATTTGATGTGGCCGAACACCTTCATACACCGAAGGAAATGGCGGCCTA
TCTCGATGCATGCATCGAAGAAAGCGACGGCGATTCGGCATTCATCGCCAAGGCGCTGGGCGATATCGCGCGTACCCAAG
GGATGAGCAAGGTCGCACGTGCGGCGGGCCTGTCGCGGGAGAGTCTGTATCGTGCCCTGTCTGGCGAGCGCAGCCCTGAT
TTCTCGACCATCCTGAAGGTGACTCGTGCCCTGGGTGTGCGCCTGCATGCCAGCGCTGCATGA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2