Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 35772..36415 Replicon plasmid pS468MPCH_P2
Accession NZ_CP079694
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC31_S468MPCH

Toxin (Protein)


Gene name vagD Uniprot ID A0A5Q9LNG2
Locus tag KW564_RS28070 Protein ID WP_048983057.1
Coordinates 35772..36188 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID R4IT22
Locus tag KW564_RS28075 Protein ID WP_016338373.1
Coordinates 36185..36415 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW564_RS28055 (KW564_28030) 30820..32928 - 2109 WP_016338368.1 peptidase domain-containing ABC transporter -
KW564_RS28060 (KW564_28035) 32918..34195 - 1278 WP_016338369.1 HlyD family secretion protein -
KW564_RS28520 34854..35390 + 537 WP_223825639.1 hypothetical protein -
KW564_RS28525 35408..35731 + 324 WP_085842301.1 hypothetical protein -
KW564_RS28070 (KW564_28045) 35772..36188 - 417 WP_048983057.1 type II toxin-antitoxin system VapC family toxin Toxin
KW564_RS28075 (KW564_28050) 36185..36415 - 231 WP_016338373.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KW564_RS28080 (KW564_28055) 36980..37198 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
KW564_RS28085 (KW564_28060) 37200..37505 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
KW564_RS28090 (KW564_28065) 37506..38312 + 807 WP_000016982.1 site-specific integrase -
KW564_RS28095 (KW564_28070) 39086..39841 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qnrB1 / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaTEM-1B / tet(D) - 1..55146 55146


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15022.52 Da        Isoelectric Point: 9.2957

>T209733 WP_048983057.1 NZ_CP079694:c36188-35772 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVK

Download         Length: 417 bp

>T209733 NZ_CP104288:c103839-102517 [Stenotrophomonas maltophilia]
ATGGGTACGCTGCAGGTCTGGATGAATGGTGTGGAAGTCGCACTTTGGGATGATGTCCGGAACGGGTCATCCCGGTTGTC
GTATGTCAGCCAGTGGATCAGCTCGCCCCAGTCCCGACCGTTGTCGCTCTCTCTACCCCTGCTCCCAGATGGCGAGAGCC
ATCGGGGCAACGTCGTCAACGACTACTTCGATAACCTTCTTCCCGATAACGTCGCCATACGCAACCGGATCCGCGACCGG
TTCGCCACGCGCGGCAGCGATACGTTCGATCTTCTTGAAGCCATTGGACGGGATTGTGTCGGCGCAGTGCAGCTGTTGCC
GCCAGCGCATCCACCCGAAGACATCCAGCAGATCACGTGCGAGGTGTTGGACGAAGCAGGTGTCGCTACGATCCTGCGTC
AGGTGACGACAGGGAACCTGCCTGGCGCACCCAGCCGTGATGATGCATTCCGCATTTCCATCGCCGGTGCGCAGGAAAAG
ACCGGTCTTCTGCTGCATGCTGGCAAATGGTGCATTCCTACCGGCAACACACCATCCACTCACATCTTCAAGCTGCCGCT
TGGCATTGTTGGCAACATGCGGGCAGACATGCATCAGTCCGTGGAGAATGAATGGCTGTGCATGCATCTGCTTTCCGCCT
TTGATATCCCCGTGGCCGAGACGCAGATGGGCCGCTTCAACGACCAACGTGCTCTCATTGTCGAGCGGTTTGACCGTCGC
CTGTCTGCCGACCGCCGGTGGTGGCTGCGACTGCCACAGGAAGACATGTGTCAGGTCTTTGGTCTGCCGGCTGCCAGAAA
GTATGAATCCGATGGCGGCCCCGGTATCGTTCCGATCATGGATCTGCTTCGACAGTCCGAACACCCCGAGGATCGCGCGA
TCTTCTTCAAGACGCAGATTCTTTTCTGGATGCTGGCCGCAACCGACGGCCATGCCAAGAATTTCAGTGTTCACCTGGAA
GCCGGTGGACGCTTCCGCCTGACGCCCGTCTACGACGTGCTGTCGGTATATCCCATCCTCGGCAGGGGACCCAATCAACT
CGATCCACGAAACGCACAGCTCGCCATGGCCGTACATGGCAAGAACCGCCACCGGAAACTGCACCTTGTGCGACGGCGGC
ACTGGAATGAAACCGCGCGCGCCTGCGGCGTTGCCGATGGTGCAGAACCATGGATCAAGACATTGATCGCGGCCGTCGAG
CCCGCCATCCAGCAGGTCACCCGTCAGCTCCCGGATGACTTCCCCTCAAACGTGTCCGAACCGATTTTCAACGGACTTCG
TGCTGCGGCAGCACGCCTTTCCAGCATGGCCCCCGAGGCTTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8751.77 Da        Isoelectric Point: 4.3533

>AT209733 WP_016338373.1 NZ_CP079694:c36415-36185 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWSSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT209733 NZ_CP104288:c104100-103843 [Stenotrophomonas maltophilia]
ATGGACAGGCCGCTGCACACAGTGAGGACAGCCCAACAACTTGCGCCCCTGATGCGTGCTTTCCGGCGCCAGGCGGGCCT
GAGCCAGGCTCAGCTGGCGGAGCGACTCGGGATCAGCCGCCAGGCAGTGGGCGCGCTGGAACGCGACCCTGCATCGGCAA
GTTTTGAACGCCTGATGCGGATCTGGGCGGTGCTGGGTCTGGAGGTCAGCCTCCAGCAACGAAGTCCCCAGGAGAACACG
TCGTCATTGGAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNG2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q9LN61

References