Detailed information of TA system TA209714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 48539..49182
• Replicon: plasmid pAI1547MPCH_P2
• Accession: NZ_CP079685
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC30_AI1547MPCH

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: C7S9Y5
• Locus tag: KW563_RS27120
• Protein ID: WP_001034046.1
• Coordinates: 48539..48955 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: V0SR71
• Locus tag: KW563_RS27125
• Protein ID: WP_001261278.1
• Coordinates: 48952..49182 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW563_RS27105 (KW563_27080)	43676..44092	-	417	WP_001034044.1	type II toxin-antitoxin system VapC family toxin	-
KW563_RS27110 (KW563_27085)	44089..44319	-	231	WP_001261286.1	type II toxin-antitoxin system VapB family antitoxin	-
KW563_RS27115 (KW563_27090)	44700..48494	+	3795	WP_001144732.1	hypothetical protein	-
KW563_RS27120 (KW563_27095)	48539..48955	-	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	Toxin
KW563_RS27125 (KW563_27100)	48952..49182	-	231	WP_001261278.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KW563_RS27130 (KW563_27105)	49447..49947	+	501	WP_000528931.1	HEPN family nuclease	-
KW563_RS27135 (KW563_27110)	49960..50733	+	774	WP_000905949.1	hypothetical protein	-
KW563_RS27140 (KW563_27115)	50900..52033	+	1134	WP_000545987.1	DUF3800 domain-containing protein	-
KW563_RS27145 (KW563_27120)	52067..52555	-	489	WP_011254646.1	hypothetical protein	-
KW563_RS27150 (KW563_27125)	53146..53364	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	-
KW563_RS27155 (KW563_27130)	53366..53671	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / sitABCD	iucA / iucB / iucC / iucD / iutA	1..71306	71306

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14978.31 Da
• Isoelectric Point: 6.7113

>T209714 WP_001034046.1 NZ_CP079685:c48955-48539 [Klebsiella pneumoniae subsp. pneumoniae]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Nucleotide

• Length: 417 bp

>T209714 NZ_CP104284:3441896-3442195 [Enterobacter hormaechei subsp. steigerwaltii]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8853.07 Da
• Isoelectric Point: 4.6437

>AT209714 WP_001261278.1 NZ_CP079685:c49182-48952 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Nucleotide

• Length: 231 bp

>AT209714 NZ_CP104284:3442206-3442571 [Enterobacter hormaechei subsp. steigerwaltii]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGCACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9NXF9

Antitoxin

• AlphaFold DB: V0SR71