Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 43676..44319 Replicon plasmid pAI1547MPCH_P2
Accession NZ_CP079685
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC30_AI1547MPCH

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag KW563_RS27105 Protein ID WP_001034044.1
Coordinates 43676..44092 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag KW563_RS27110 Protein ID WP_001261286.1
Coordinates 44089..44319 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW563_RS27090 (KW563_27065) 40078..40775 + 698 WP_223155668.1 IS1-like element IS1A family transposase -
KW563_RS27095 (KW563_27070) 41029..42051 - 1023 WP_000361402.1 helicase UvrD -
KW563_RS27100 (KW563_27075) 42036..43601 - 1566 WP_001128474.1 AAA family ATPase -
KW563_RS27105 (KW563_27080) 43676..44092 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
KW563_RS27110 (KW563_27085) 44089..44319 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KW563_RS27115 (KW563_27090) 44700..48494 + 3795 WP_001144732.1 hypothetical protein -
KW563_RS27120 (KW563_27095) 48539..48955 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
KW563_RS27125 (KW563_27100) 48952..49182 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / sitABCD iucA / iucB / iucC / iucD / iutA 1..71306 71306


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T209713 WP_001034044.1 NZ_CP079685:c44092-43676 [Klebsiella pneumoniae subsp. pneumoniae]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T209713 NZ_CP104284:c2645843-2645740 [Enterobacter hormaechei subsp. steigerwaltii]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT209713 WP_001261286.1 NZ_CP079685:c44319-44089 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT209713 NZ_CP104284:2645727-2645936 [Enterobacter hormaechei subsp. steigerwaltii]
TTTTCAAGCAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGC
TAAAAGTTGGCATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGCA
AAAGTGGTCTGAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References