Detailed information of TA system TA209712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5299430..5300055
• Replicon: chromosome
• Accession: NZ_CP079683
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC30_AI1547MPCH

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: KW563_RS25790
• Protein ID: WP_002882817.1
• Coordinates: 5299430..5299813 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: KW563_RS25795
• Protein ID: WP_004150355.1
• Coordinates: 5299813..5300055 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW563_RS25775 (5296796)	5296796..5297698	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
KW563_RS25780 (5297695)	5297695..5298330	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
KW563_RS25785 (5298327)	5298327..5299256	+	930	WP_064162495.1	formate dehydrogenase accessory protein FdhE	-
KW563_RS25790 (5299430)	5299430..5299813	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
KW563_RS25795 (5299813)	5299813..5300055	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
KW563_RS25800 (5300260)	5300260..5301177	+	918	WP_004181612.1	alpha/beta hydrolase	-
KW563_RS25805 (5301191)	5301191..5302132	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
KW563_RS25810 (5302177)	5302177..5302614	-	438	WP_064162494.1	D-aminoacyl-tRNA deacylase	-
KW563_RS25815 (5302611)	5302611..5303471	-	861	WP_004146232.1	virulence factor BrkB family protein	-
KW563_RS25820 (5303465)	5303465..5304064	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T209712 WP_002882817.1 NZ_CP079683:c5299813-5299430 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T209712 NZ_CP104284:c2239908-2239423 [Enterobacter hormaechei subsp. steigerwaltii]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATACCCGTGATTATACTTGCCCGTCTGGCTGTGGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATTATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCTTCGCAGACTCAGGAACGGACACTGTTTCTCCGGCTTCCC
CAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT209712 WP_004150355.1 NZ_CP079683:c5300055-5299813 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT209712 NZ_CP104284:c2240162-2239896 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCACAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8

Antitoxin

• AlphaFold DB: A0A0H3GGU9