Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4074638..4075257 Replicon chromosome
Accession NZ_CP079683
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC30_AI1547MPCH

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KW563_RS19975 Protein ID WP_002892050.1
Coordinates 4075039..4075257 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KW563_RS19970 Protein ID WP_002892066.1
Coordinates 4074638..4075012 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW563_RS19960 (4069790) 4069790..4070983 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KW563_RS19965 (4071006) 4071006..4074152 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KW563_RS19970 (4074638) 4074638..4075012 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KW563_RS19975 (4075039) 4075039..4075257 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KW563_RS19980 (4075420) 4075420..4075986 + 567 WP_002892030.1 maltose O-acetyltransferase -
KW563_RS19985 (4075958) 4075958..4076098 - 141 WP_004147370.1 hypothetical protein -
KW563_RS19990 (4076119) 4076119..4076589 + 471 WP_002892026.1 YlaC family protein -
KW563_RS19995 (4076564) 4076564..4078015 - 1452 WP_064162152.1 PLP-dependent aminotransferase family protein -
KW563_RS20000 (4078116) 4078116..4078814 + 699 WP_004177238.1 GNAT family protein -
KW563_RS20005 (4078811) 4078811..4078951 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KW563_RS20010 (4078951) 4078951..4079214 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T209709 WP_002892050.1 NZ_CP079683:4075039-4075257 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T209709 NZ_CP104284:c1075298-1075080 [Enterobacter hormaechei subsp. steigerwaltii]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTTGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAGC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTGTGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT209709 WP_002892066.1 NZ_CP079683:4074638-4075012 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT209709 NZ_CP104284:c1075700-1075326 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References