Detailed information of TA system TA020970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 2290113..2290702
• Replicon: chromosome
• Accession: NC_004757
• Organism: Nitrosomonas europaea ATCC 19718

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: ALW85_RS13745
• Protein ID: WP_198407885.1
• Coordinates: 2290113..2290355 (-)
• Length: 81 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q82T28
• Locus tag: ALW85_RS10965
• Protein ID: WP_011112611.1
• Coordinates: 2290451..2290702 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ALW85_RS14220	2285457..2285603	+	147	WP_158297474.1	hypothetical protein	-
ALW85_RS10935 (NE2098)	2285699..2287000	+	1302	WP_198407847.1	group II intron reverse transcriptase/maturase	-
ALW85_RS10940 (RK28_RS10920)	2287101..2287331	-	231	WP_041356832.1	hypothetical protein	-
ALW85_RS10945 (NE2099)	2287493..2287999	-	507	WP_041356833.1	TIR domain-containing protein	-
ALW85_RS10950 (NE2100)	2288019..2288435	-	417	WP_041356834.1	hypothetical protein	-
ALW85_RS10955 (RK28_RS10935)	2288831..2289037	+	207	WP_041356835.1	hypothetical protein	-
ALW85_RS13740	2289234..2289694	+	461	Protein_2117	DDE-type integrase/transposase/recombinase	-
ALW85_RS10960 (NE2102)	2289895..2290104	-	210	WP_041357279.1	BrnA antitoxin family protein	-
ALW85_RS13745	2290113..2290355	-	243	WP_198407885.1	BrnT family toxin	Toxin
ALW85_RS10965 (NE2103)	2290451..2290702	-	252	WP_011112611.1	CopG family transcriptional regulator	Antitoxin
ALW85_RS10970 (NE2104)	2290692..2290955	-	264	WP_011112612.1	BrnT family toxin	-
ALW85_RS10975 (RK28_RS10955)	2291080..2291274	-	195	WP_041356836.1	hypothetical protein	-
ALW85_RS10985 (NE2106)	2292041..2292427	+	387	WP_041356838.1	DUF2384 domain-containing protein	-
ALW85_RS10990 (NE2107)	2292427..2292885	+	459	WP_011112615.1	RES family NAD+ phosphorylase	-
ALW85_RS11005 (RK28_RS10985)	2294322..2294531	-	210	WP_041356839.1	addiction module protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	2284323..2291274	6951

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (2-75)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 81 a.a.
• Molecular weight: 9275.49 Da
• Isoelectric Point: 9.1077

>T20970 WP_198407885.1 NC_004757:c2290355-2290113 [Nitrosomonas europaea ATCC 19718]
IQNLRKHGVSFEEAASVFSDERARLIDDPDHSAEEERFVLLGFSANLRVLVVVHCYRRSGSTIRLISARKATREERRFYP

Nucleotide

• Length: 243 bp

>T20970 NC_004757:c2290355-2290113 [Nitrosomonas europaea ATCC 19718]
ATCCAGAACCTGCGCAAGCATGGTGTTTCGTTCGAAGAGGCTGCGTCAGTGTTCTCCGACGAGCGAGCTCGCCTCATTGA
CGATCCCGACCATTCGGCTGAGGAGGAACGGTTCGTCTTGCTGGGTTTCAGCGCGAATCTTCGAGTCCTTGTCGTGGTTC
ATTGTTACCGGAGATCTGGAAGCACGATTCGCCTTATTTCCGCCCGGAAGGCGACACGAGAGGAGAGGCGGTTTTACCCA
TAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9515.87 Da
• Isoelectric Point: 7.0158

>AT20970 WP_011112611.1 NC_004757:c2290702-2290451 [Nitrosomonas europaea ATCC 19718]
MKAKDFEQQFDEGVDITASLDLSKAKRVLQEQKRVNVDFPTWMIESLDREAEKLGVTRQSIIKVWLAERLEKAALTHPSS
GTR

Nucleotide

• Length: 252 bp

>AT20970 NC_004757:c2290702-2290451 [Nitrosomonas europaea ATCC 19718]
ATGAAAGCTAAAGATTTTGAACAGCAGTTTGACGAAGGCGTTGATATTACTGCTTCGCTGGATTTGTCAAAGGCAAAGCG
CGTATTGCAAGAGCAAAAACGTGTCAATGTCGATTTCCCAACGTGGATGATTGAGTCTCTTGACCGGGAAGCTGAAAAGC
TTGGTGTGACCAGGCAATCTATCATTAAGGTGTGGCTTGCCGAACGCCTCGAAAAGGCTGCTCTAACCCATCCATCAAGC
GGGACGCGCTAA

Structures

Antitoxin

• AlphaFold DB: Q82T28