Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 58669..59405 Replicon plasmid pCMC309MPCH_P1
Accession NZ_CP079674
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC29_CMC309MPCH

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KW562_RS25065 Protein ID WP_003026803.1
Coordinates 58923..59405 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KW562_RS25060 Protein ID WP_003026799.1
Coordinates 58669..58935 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW562_RS25015 (KW562_25005) 54731..55093 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
KW562_RS25020 (KW562_25010) 55143..55493 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KW562_RS25025 (KW562_25015) 55851..56120 + 270 WP_004152102.1 hypothetical protein -
KW562_RS25030 (KW562_25020) 56108..56683 + 576 WP_004152103.1 hypothetical protein -
KW562_RS25035 (KW562_25025) 56714..57208 + 495 WP_004152104.1 DNA-binding protein -
KW562_RS25040 (KW562_25030) 57252..57620 + 369 WP_004152105.1 hypothetical protein -
KW562_RS25045 (KW562_25035) 57654..57857 + 204 WP_004152106.1 HHA domain-containing protein -
KW562_RS25050 (KW562_25040) 57906..58163 + 258 WP_004152107.1 hypothetical protein -
KW562_RS25055 (KW562_25045) 58239..58493 + 255 WP_004152108.1 hypothetical protein -
KW562_RS25060 (KW562_25050) 58669..58935 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KW562_RS25065 (KW562_25055) 58923..59405 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KW562_RS25070 (KW562_25060) 59617..60963 + 1347 WP_020314316.1 ISNCY family transposase -
KW562_RS27230 61124..61255 + 132 WP_256357619.1 hypothetical protein -
KW562_RS25075 (KW562_25065) 61464..62629 - 1166 Protein_67 IS3 family transposase -
KW562_RS25080 (KW562_25070) 62806..63768 - 963 WP_004152113.1 zinc metalloprotease HtpX -
KW562_RS25085 (KW562_25075) 63755..64243 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 - 1..213013 213013


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T209696 WP_003026803.1 NZ_CP079674:58923-59405 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T209696 NZ_CP104274:3572673-3572891 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT209696 WP_003026799.1 NZ_CP079674:58669-58935 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT209696 NZ_CP104274:3572273-3572647 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References