Detailed information of TA system TA209695

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4706239..4707827
• Replicon: chromosome
• Accession: NZ_CP079673
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC29_CMC309MPCH

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KW562_RS22490
• Protein ID: WP_004179815.1
• Coordinates: 4706505..4707827 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KW562_RS22485
• Protein ID: WP_004176198.1
• Coordinates: 4706239..4706505 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW562_RS22460 (4701389)	4701389..4702312	+	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-
KW562_RS22465 (4702293)	4702293..4703447	-	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
KW562_RS22470 (4703793)	4703793..4705172	+	1380	WP_004179808.1	nucleobase:cation symporter-2 family protein	-
KW562_RS22475 (4705150)	4705150..4705650	+	501	WP_004179811.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KW562_RS22480 (4705647)	4705647..4705973	+	327	WP_002904788.1	hydroxyisourate hydrolase	-
KW562_RS22485 (4706239)	4706239..4706505	+	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KW562_RS22490 (4706505)	4706505..4707827	+	1323	WP_004179815.1	type II toxin-antitoxin system HipA family toxin	Toxin
KW562_RS22495 (4707844)	4707844..4709136	-	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
KW562_RS22500 (4709278)	4709278..4710282	-	1005	WP_218394504.1	fimbrial protein	-
KW562_RS22505 (4710270)	4710270..4712771	-	2502	WP_004179823.1	fimbria/pilus outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49152.42 Da
• Isoelectric Point: 7.2850

>T209695 WP_004179815.1 NZ_CP079673:4706505-4707827 [Klebsiella pneumoniae subsp. pneumoniae]
MATLTTWMNNVRVGTLTHQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T209695 NZ_CP104274:c3544888-3544586 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT209695 WP_004176198.1 NZ_CP079673:4706239-4706505 [Klebsiella pneumoniae subsp. pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT209695 NZ_CP104274:c3544550-3544209 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG