Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3369696..3370315 Replicon chromosome
Accession NZ_CP079673
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC29_CMC309MPCH

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KW562_RS16150 Protein ID WP_002892050.1
Coordinates 3369696..3369914 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KW562_RS16155 Protein ID WP_002892066.1
Coordinates 3369941..3370315 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW562_RS16115 (3365743) 3365743..3366006 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
KW562_RS16120 (3366006) 3366006..3366146 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KW562_RS16125 (3366143) 3366143..3366841 - 699 WP_004178762.1 GNAT family protein -
KW562_RS16130 (3366942) 3366942..3368393 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KW562_RS16135 (3368368) 3368368..3368838 - 471 WP_002892026.1 YlaC family protein -
KW562_RS16140 (3368859) 3368859..3368999 + 141 WP_004147370.1 hypothetical protein -
KW562_RS16145 (3368971) 3368971..3369537 - 567 WP_002892030.1 maltose O-acetyltransferase -
KW562_RS16150 (3369696) 3369696..3369914 - 219 WP_002892050.1 HHA domain-containing protein Toxin
KW562_RS16155 (3369941) 3369941..3370315 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KW562_RS16160 (3370801) 3370801..3373947 - 3147 WP_117248583.1 multidrug efflux RND transporter permease subunit AcrB -
KW562_RS16165 (3373970) 3373970..3375163 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3364637..3365683 1046


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T209693 WP_002892050.1 NZ_CP079673:c3369914-3369696 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T209693 NZ_CP104274:2467716-2467994 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT209693 WP_002892066.1 NZ_CP079673:c3370315-3369941 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT209693 NZ_CP104274:2467994-2468278 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References