Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2773841..2774651 Replicon chromosome
Accession NZ_CP079673
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC29_CMC309MPCH

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KW562_RS13320 Protein ID WP_004178461.1
Coordinates 2774118..2774651 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KW562_RS13315 Protein ID WP_002887278.1
Coordinates 2773841..2774107 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW562_RS13295 (2769713) 2769713..2770057 - 345 WP_004197273.1 cation efflux system protein CusF -
KW562_RS13300 (2770075) 2770075..2771460 - 1386 WP_004178457.1 efflux transporter outer membrane subunit -
KW562_RS13305 (2771632) 2771632..2772315 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
KW562_RS13310 (2772305) 2772305..2773738 + 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
KW562_RS13315 (2773841) 2773841..2774107 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KW562_RS13320 (2774118) 2774118..2774651 + 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KW562_RS13325 (2774699) 2774699..2775820 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T209692 WP_004178461.1 NZ_CP079673:2774118-2774651 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T209692 NZ_CP104274:2134253-2134355 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT209692 WP_002887278.1 NZ_CP079673:2773841-2774107 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT209692 NZ_CP104274:c2134393-2134125 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References