Detailed information of TA system TA209687

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 1285194..1285851
• Replicon: chromosome
• Accession: NZ_CP079673
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC29_CMC309MPCH

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: KW562_RS06185
• Protein ID: WP_004181233.1
• Coordinates: 1285194..1285604 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: KW562_RS06190
• Protein ID: WP_002916312.1
• Coordinates: 1285585..1285851 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW562_RS06165 (1281194)	1281194..1282927	-	1734	WP_004149758.1	single-stranded-DNA-specific exonuclease RecJ	-
KW562_RS06170 (1282933)	1282933..1283646	-	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
KW562_RS06175 (1283669)	1283669..1284565	-	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
KW562_RS06180 (1284666)	1284666..1285187	+	522	WP_004144730.1	flavodoxin FldB	-
KW562_RS06185 (1285194)	1285194..1285604	-	411	WP_004181233.1	protein YgfX	Toxin
KW562_RS06190 (1285585)	1285585..1285851	-	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
KW562_RS06195 (1286097)	1286097..1287080	+	984	WP_002916313.1	tRNA-modifying protein YgfZ	-
KW562_RS06200 (1287231)	1287231..1287890	-	660	WP_002916317.1	hemolysin III family protein	-
KW562_RS06205 (1288054)	1288054..1288365	-	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
KW562_RS06210 (1288415)	1288415..1289143	+	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
KW562_RS06215 (1289262)	1289262..1290695	+	1434	WP_004181234.1	6-phospho-beta-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16121.92 Da
• Isoelectric Point: 11.1565

>T209687 WP_004181233.1 NZ_CP079673:c1285604-1285194 [Klebsiella pneumoniae subsp. pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAEEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T209687 NZ_CP104274:726763-727059 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT209687 WP_002916312.1 NZ_CP079673:c1285851-1285585 [Klebsiella pneumoniae subsp. pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT209687 NZ_CP104274:727061-727456 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GY41