Detailed information of TA system TA020968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 1695137..1695668
• Replicon: chromosome
• Accession: NC_004757
• Organism: Nitrosomonas europaea ATCC 19718

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q82UC9
• Locus tag: ALW85_RS08155
• Protein ID: WP_011112127.1
• Coordinates: 1695378..1695668 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q82UD0
• Locus tag: ALW85_RS08150
• Protein ID: WP_011112126.1
• Coordinates: 1695137..1695388 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ALW85_RS14005 (NE1554)	1691222..1691524	+	303	WP_143023767.1	hypothetical protein	-
ALW85_RS08115 (NE1555)	1691603..1692085	+	483	WP_143023768.1	hypothetical protein	-
ALW85_RS08120 (NE1556)	1692108..1692632	+	525	WP_011112120.1	hypothetical protein	-
ALW85_RS08125 (NE1557)	1692646..1693005	+	360	WP_011112121.1	IS630 transposase-related protein	-
ALW85_RS08130 (NE1558)	1693067..1693480	+	414	WP_011112122.1	transposase	-
ALW85_RS08135 (NE1559)	1693557..1693973	+	417	WP_011112123.1	hypothetical protein	-
ALW85_RS08140 (NE1560)	1694061..1694564	+	504	WP_011112124.1	DNA-deoxyinosine glycosylase	-
ALW85_RS08145 (NE1561)	1694663..1694980	+	318	WP_011112125.1	hypothetical protein	-
ALW85_RS08150 (NE1562)	1695137..1695388	+	252	WP_011112126.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
ALW85_RS08155 (NE1563)	1695378..1695668	+	291	WP_011112127.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ALW85_RS08160 (NE1564)	1695728..1696417	+	690	WP_143023770.1	adenine nucleotide alpha hydrolase	-
ALW85_RS08165 (NE1565)	1696712..1698025	+	1314	WP_011112129.1	hypothetical protein	-
ALW85_RS08170 (NE1566)	1698144..1699199	+	1056	WP_143023771.1	hypothetical protein	-
ALW85_RS08175 (NE1567)	1699292..1700128	-	837	WP_011112131.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1690373..1690726	353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-71)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11320.26 Da
• Isoelectric Point: 10.6491

>T20968 WP_011112127.1 NC_004757:1695378-1695668 [Nitrosomonas europaea ATCC 19718]
MTYKLKFLPSAKKEWDKLDSSIKTQFKNKLKKCLENPHIQPNKLRGFDNAYKIKLRSAGYRLVYEINNQEVVVFVIAVGK
RENNKIYDKAINRTKT

Nucleotide

• Length: 291 bp

>T20968 NC_004757:1695378-1695668 [Nitrosomonas europaea ATCC 19718]
ATGACTTATAAATTAAAATTTTTGCCAAGTGCAAAAAAAGAGTGGGATAAACTGGACAGCAGCATCAAAACCCAATTTAA
AAACAAATTAAAAAAATGCCTGGAAAATCCTCATATTCAGCCTAATAAACTACGTGGTTTTGATAACGCTTATAAAATCA
AACTCCGCTCTGCAGGTTATCGGCTTGTTTATGAAATCAATAATCAGGAAGTCGTTGTTTTCGTTATCGCTGTAGGCAAA
AGGGAAAATAATAAGATTTACGATAAAGCAATCAACAGAACCAAAACTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9288.72 Da
• Isoelectric Point: 4.6337

>AT20968 WP_011112126.1 NC_004757:1695137-1695388 [Nitrosomonas europaea ATCC 19718]
MHQILASFSASISELKKNPTALLRKAEGETIAILNHNLPTAYLVPAEVYELLMEKLEDYELGEIVKARQAEKHLAIEVSL
DDL

Nucleotide

• Length: 252 bp

>AT20968 NC_004757:1695137-1695388 [Nitrosomonas europaea ATCC 19718]
ATGCACCAAATACTGGCTTCATTTAGCGCAAGCATCTCTGAATTAAAGAAAAATCCAACAGCTTTGTTAAGAAAAGCCGA
AGGGGAAACTATCGCTATTCTCAACCATAACCTGCCAACCGCTTACCTTGTTCCAGCCGAGGTATACGAGTTGTTGATGG
AGAAATTGGAAGATTATGAATTAGGCGAAATTGTTAAGGCCAGGCAAGCGGAAAAACATTTGGCAATTGAAGTATCGCTT
GATGACTTATAA

Structures

Toxin

• AlphaFold DB: Q82UC9

Antitoxin

• AlphaFold DB: Q82UD0