Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 58669..59405 Replicon plasmid pCMC387MPCH_P2
Accession NZ_CP079670
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC28_CMC387MPCH

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KW561_RS28215 Protein ID WP_003026803.1
Coordinates 58923..59405 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KW561_RS28210 Protein ID WP_003026799.1
Coordinates 58669..58935 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW561_RS28165 (KW561_28150) 54731..55093 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
KW561_RS28170 (KW561_28155) 55143..55493 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KW561_RS28175 (KW561_28160) 55851..56120 + 270 WP_004152102.1 hypothetical protein -
KW561_RS28180 (KW561_28165) 56108..56683 + 576 WP_004152103.1 hypothetical protein -
KW561_RS28185 (KW561_28170) 56714..57208 + 495 WP_004152104.1 DNA-binding protein -
KW561_RS28190 (KW561_28175) 57252..57620 + 369 WP_004152105.1 hypothetical protein -
KW561_RS28195 (KW561_28180) 57654..57857 + 204 WP_004152106.1 HHA domain-containing protein -
KW561_RS28200 (KW561_28185) 57906..58163 + 258 WP_004152107.1 hypothetical protein -
KW561_RS28205 (KW561_28190) 58239..58493 + 255 WP_004152108.1 hypothetical protein -
KW561_RS28210 (KW561_28195) 58669..58935 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KW561_RS28215 (KW561_28200) 58923..59405 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KW561_RS28220 (KW561_28205) 59617..60963 + 1347 WP_020314316.1 ISNCY family transposase -
KW561_RS29380 61124..61255 + 132 WP_004218042.1 hypothetical protein -
KW561_RS28225 (KW561_28210) 61464..62629 - 1166 Protein_67 IS3 family transposase -
KW561_RS28230 (KW561_28215) 62806..63768 - 963 WP_004152113.1 zinc metalloprotease HtpX -
KW561_RS28235 (KW561_28220) 63755..64243 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 - 1..211813 211813


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T209678 WP_003026803.1 NZ_CP079670:58923-59405 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T209678 NZ_CP104254:615817-615999 [Pseudomonas aeruginosa]
ATGCGGAGTAGGGAAGTGATTGATTTGCTGTTGGAGGATGGCTGGTATGAAGTGGCGGTGAAAGGTAGCCACCACCAGTT
CAAACATCCTTCAAAGCCTGGAAAGGTGACGGTTCAACATCCGTCCTCGACGATACCGAAAGGAACCCTGAACAACATAC
TGAAACAAGCCGGCCTCAAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT209678 WP_003026799.1 NZ_CP079670:58669-58935 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT209678 NZ_CP104254:616027-616431 [Pseudomonas aeruginosa]
ATGAAATTCCCCGTGGTGTTACACAAAGACCCTGATTCTGACTATGGCGTGACTGTGCCCGATGTGCCGGGTTGCTTCTC
TGCCGGCGCTACGGTGTCCGAGGCCCTGGCCAATGTAGAAGAGGCGCTGGCCTTGCACTTCGAAGGCTTGGTTACGGACG
GCGAAGAGCTACCCCAGCCGCAGGACGTTGACGCCCATATGAAGAATCCGGACTTCGAAGGTGGCGTATGGGCGGTGGTT
GACTTCGACGTGACCCCGTACCTGGGCAAGGCCGTACGCTTTAATGCGACGCTCCCGGAACACCTTCTGCAGCGGATCGA
TGAGCGCGTGAAGGTGGATAAGCGCTATCAGTCTCGGTCGGGCTTTCTGGCAACAGCGGCGATGCGCGAACTGTCAGTGG
CGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References