Detailed information of TA system TA209675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 23055..23577
• Replicon: plasmid pCMC387MPCH_P1
• Accession: NZ_CP079669
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC28_CMC387MPCH

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A0C7KG00
• Locus tag: KW561_RS26515
• Protein ID: WP_038991638.1
• Coordinates: 23293..23577 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4R0U8
• Locus tag: KW561_RS26510
• Protein ID: WP_004181777.1
• Coordinates: 23055..23303 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW561_RS26490 (KW561_26475)	18264..19085	-	822	WP_004181772.1	hypothetical protein	-
KW561_RS26495 (KW561_26480)	19147..19500	-	354	WP_004181774.1	hypothetical protein	-
KW561_RS26500 (KW561_26485)	19645..20631	-	987	WP_040120328.1	hypothetical protein	-
KW561_RS26505 (KW561_26490)	20965..22764	-	1800	WP_043907032.1	ATP-dependent helicase	-
KW561_RS26510 (KW561_26495)	23055..23303	+	249	WP_004181777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KW561_RS26515 (KW561_26500)	23293..23577	+	285	WP_038991638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KW561_RS26520 (KW561_26505)	23594..23695	-	102	Protein_27	IS200/IS605 family transposase	-
KW561_RS26525 (KW561_26510)	23731..24957	+	1227	WP_040120327.1	RNA-guided endonuclease TnpB family protein	-
KW561_RS26530 (KW561_26515)	25229..25453	-	225	Protein_29	transposase	-
KW561_RS26535 (KW561_26520)	25532..25960	-	429	WP_077256229.1	IS200/IS605 family transposase	-
KW561_RS26540 (KW561_26525)	25996..27183	+	1188	WP_043907031.1	RNA-guided endonuclease TnpB family protein	-
KW561_RS29235	27228..27599	-	372	WP_044816264.1	hypothetical protein	-
KW561_RS29240	27596..27940	-	345	WP_014386518.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-15 / aac(6')-Ib / blaTEM-1A / tet(D) / dfrA14 / qnrB1 / blaOXA-1 / aac(6')-Ib-cr / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12	-	1..286022	286022

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10993.73 Da
• Isoelectric Point: 10.6516

>T209675 WP_038991638.1 NZ_CP079669:23293-23577 [Klebsiella pneumoniae subsp. pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Nucleotide

• Length: 285 bp

>T209675 NZ_CP104215:c3318662-3318384 [Burkholderia gladioli]
ATGATCCAGTCATTCCGATGCAAGCGCACCGCCGCGCTGTTCGCCGGCGAATCGCCGCCGGCCTGGCGTGCCGTGGCACA
AGTGGCCCGGCGCAAGCTGGCCATGCTCGATGCGGCCCAGGCGCTGCGCGACCTGGCCGTGCCGCCCGGCAACCAACTCG
AAGGCCTGTTCGGCGATCGCGCGGGGCAATACAGCATCCGGATCAACGAGCGCTGGCGCCTTTGTTTCGTCTGGAGCAGC
CGGGGGCCCGAGCTCGTCGAGATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8925.17 Da
• Isoelectric Point: 3.8209

>AT209675 WP_004181777.1 NZ_CP079669:23055-23303 [Klebsiella pneumoniae subsp. pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT209675 NZ_CP104215:c3318365-3318042 [Burkholderia gladioli]
ATGAATGCCAAGAGTGTGAAGCCCGTCAACGCGATGCCCGCCATCCACCCCGGCGAAATCCTGCGCGAGGAGTTCCTGGT
GCCGCTCGCGCTCAGCGTCAACGCGCTGTCGCTGGCGCTGATGGTGCCGGCCACCCGCATGCACGAGATCGTCAACGAGC
GGCGCGGCATCACCGCCGACACGGCCTACCGCCTGTCGCGCTACTTCGGCACCACGCCGGAGTTCTGGCTGGCCCTGCAG
GCCGACTACGACATCAAGACCCTGCCGAACCGCGACGAGATCGACCGCAAGGTCGATGCGCGCCCCAACGGCGCGGCGGC
CTGA

Structures

Toxin

• AlphaFold DB: A0A0C7KG00

Antitoxin

• AlphaFold DB: A0A2J4R0U8