209674

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5364816..5365441 Replicon chromosome
Accession NZ_CP079668
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC28_CMC387MPCH

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KW561_RS25935 Protein ID WP_002882817.1
Coordinates 5364816..5365199 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KW561_RS25940 Protein ID WP_004150355.1
Coordinates 5365199..5365441 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW561_RS25920 (5362182) 5362182..5363084 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KW561_RS25925 (5363081) 5363081..5363716 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KW561_RS25930 (5363713) 5363713..5364642 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KW561_RS25935 (5364816) 5364816..5365199 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KW561_RS25940 (5365199) 5365199..5365441 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KW561_RS25945 (5365646) 5365646..5366563 + 918 WP_004181612.1 alpha/beta hydrolase -
KW561_RS25950 (5366577) 5366577..5367518 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KW561_RS25955 (5367563) 5367563..5368000 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KW561_RS25960 (5367997) 5367997..5368857 - 861 WP_004146232.1 virulence factor BrkB family protein -
KW561_RS25965 (5368851) 5368851..5369450 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209674 WP_002882817.1 NZ_CP079668:c5365199-5364816 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209674 NZ_CP104215:2933634-2934599 [Burkholderia gladioli]
ATGAGCGAAATCGATCCGCGCGGGGCCGAGGCAGAGGCCGGGCCCGACGCGGCCCTGGCCGCCCGGCTCGGGCAGTTCCC
GGCGCGGCGCATCGCCATTGGCGAAGCCGGGCTGATCGGCTGGCGCGAATCGGGTCCAGCGCAGGGGGCCGGCGATTCGA
CGGCACGCGCGGCCGCTGGCGAGCGGGCATTGCCGGTGGTGCTGCTGCACGGCATCGGCTCGGGCGCGGCCTCGTGGCTG
GCCCAGCTCGACACGCTCGGCGCCTCGCGTCGCGTGCTGGCCTGGGACGCGCCCGGTTATGGCGAATCGACGCCGGTCGC
GCCGGCCTCGCCGCTGGCGGCCGATTACGCGGCGTCGCTGGCCGGCTGGCTCGACGCGCTCGCCATCGAGCGCTGCGTGC
TGGTGGGGCACTCGCTCGGCGCGATCGTGGCCGGCGCCTTCGCGCGCCGGCATGCCGGGCGGCTGGCCGGCTTGCTGCTG
GTTTCGCCGGCCGCCGGCTACGGCGCGGCGCCGGCCGCCACGCGCGACGAACGGCGCGATGCGCGGCTCGCGATGCTGGC
CGCGCTCGGGCCCGCGGGCCTGGCCGAACAACGCAGCGCCAACATGCTGTCGGCGCATGCCGGCGAGGCCGCCCGCGCCT
GGGTGCGCCGCGTGATGGCGCAGGTGAAACCGGCCGGCTACACGCAGGCCACCCACCTGCTCGCGAACGCCGACCTGGCC
GCCGATCTCGCCGCCTGGCGGGCGATGACCGACGCCGCCGGTGCCGCGCGCGTGGAGGTGATGGTGGGCGCCGAGGACGG
CATCACGCCGCCGAACGCCTGCGAACGGATCGCGGCGGCGGCCGGCGTGGCGCTGCGTATCGTGCCGCGCGCCGGCCATG
CCGGCTACGTGGAGGCACCCGAGCGCTACTCGGCGCTGATCGGCGCGTTCTGCGCGCGCTGCGACGAATCATGGAGACAG
GCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209674 WP_004150355.1 NZ_CP079668:c5365441-5365199 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209674 NZ_CP104215:2934596-2935477 [Burkholderia gladioli]
ATGAACCCGACCCAACTCGCCCTGCGAAGCGAGCCGGCCGACAGCGGCGCGCCCGAGGCGGATGCCGCGAACGGCGCCTA
CGTGGTACCTGGCCTCGAACGCGGGCTGCGGATCCTCGCGGAATTCTCGCCGCGCGAGCCGGTGCTGGGCGCGCCGGAAT
TGTCGAAGCGGCTCGGCATTCCGCGCACCACGGTGTTCCGGCTGCTGCAGACGCTCGAATCGCTGGGCTTCCTGGAGCGC
GCCGACAAGGACCGCAATTACCGGCTCGGCGTGGCGGTGCTGCGCCTGGGCTTCGAATACTTGAGCTCGCTGGAGCTGAC
CGACCTGGGCCTGCCGGTGATCGAGGGCCTGCGCGAGGCGACCGGCTTCACCACCCATATCGTGATCCGCGACGGCCGCG
ACGTGGTGTTCGTCGCCAAGGCGCAGAGCGCGGCGCCGGCCTTCAGCTCGATTCGCGTCAACGTCGGCACGCGGCTGCCC
GCGCATGCCACCACCCACGGCCACGTGCTGATGGGCGACCTGAGCGAGGCCGAGCTGCGCGCGCTGTACCCCGAGCGCCA
GCTCGAACGCGCCACGCGTGCCACGCCCGACAGCCTGGAGGCGCTGCTCGCGCTGGTCCGCGAGGATGCCGCGCGCGGCT
ACGGCATCAGCAACGCCTCGTTCGAGCGCGGCATCTCGGTGGTCACCGCGCCGGTGCGCAACGACACGCAGCGCATCGTG
GCGGCCGTCACGGTGACGGTGCCGAAACCCGAGATCGACGCGGCGCTGATCGCCGACGGCCTGGTCGAGAAGGTCGTGAG
GGCGGCCAATGAACTGTCGCGGCGGCTCAATTACCGCTCCGATGATGAGAACTCCTATCTGAAGGCATTGGGATTGCGAT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References