Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4785794..4786604 Replicon chromosome
Accession NZ_CP079668
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC28_CMC387MPCH

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KW561_RS23255 Protein ID WP_004178461.1
Coordinates 4785794..4786327 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KW561_RS23260 Protein ID WP_002887278.1
Coordinates 4786338..4786604 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW561_RS23250 (4784625) 4784625..4785746 + 1122 WP_002887282.1 cupin domain-containing protein -
KW561_RS23255 (4785794) 4785794..4786327 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KW561_RS23260 (4786338) 4786338..4786604 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KW561_RS23265 (4786707) 4786707..4788140 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
KW561_RS23270 (4788130) 4788130..4788813 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KW561_RS23275 (4788985) 4788985..4790370 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
KW561_RS23280 (4790388) 4790388..4790732 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T209671 WP_004178461.1 NZ_CP079668:c4786327-4785794 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T209671 NZ_CP104215:c112720-112298 [Burkholderia gladioli]
ATGTATTTGATTGACACCAATGTGGTCAGCGAAGTCCGGAAACGGGATCGTGCGGACAAGGGCGTGATGGCCTTCTTTCG
CCAGGCGGCCCGCGACGATGCCGGCCTGTACCTGTCGGTGGTGACGGTGGGCGAACTGCGGCGAGGTGTGGAAATCATCC
GTCACCGTGGCGACAAATCCCAGGCAACGCGTCTCGCGAACTGGCTCGATGGCGTGCTCCGGGAGTTTGCTGAGAACATT
CTCGTGGTGGACAAGGAGATCGGGCAGATGTGGGGCTACCTGCGTGTGCCTCGCTCGGAACACTCGCTGGATAAGGTGAT
CGCCGCCACGGCCTTGATTCACGATCTGACCGTGGTGACGCGCAACGTGGATGATTTCGCCGGAACCGGTGCCCGTGTGC
TGAACCCGTTCAAGGGACCATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT209671 WP_002887278.1 NZ_CP079668:c4786604-4786338 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT209671 NZ_CP104215:c112940-112707 [Burkholderia gladioli]
ATGGCGAATCTTCTGGTGAGAAACGTGGACGAAATGCTTGTTCAAATCTTGCGCGAGCGGGCTGCCGCTCACGGGCACAG
CGCGGAAGCCGAGCATCGTGCAATCCTGGCAAGGGCGCTGCGCGAGCCGCAGCGCAAGACATTCGCGCAAGTGCTGATGG
GCATGCCGAACGTCGGTAGCGATGTCGATTTTGCGCGCGTGGACGATGGCGAGGCGGCGAATGTATTTGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References