Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 6883..7526 Replicon plasmid pS471M_P3
Accession NZ_CP079658
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC27_S471MPC

Toxin (Protein)


Gene name vagD Uniprot ID A0A5Q9LNG2
Locus tag KW560_RS28790 Protein ID WP_048983057.1
Coordinates 6883..7299 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID R4IT22
Locus tag KW560_RS28795 Protein ID WP_016338373.1
Coordinates 7296..7526 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW560_RS28775 (KW560_28750) 1931..4039 - 2109 WP_016338368.1 peptidase domain-containing ABC transporter -
KW560_RS28780 (KW560_28755) 4029..5306 - 1278 WP_016338369.1 HlyD family secretion protein -
KW560_RS29440 5965..6501 + 537 WP_223825639.1 hypothetical protein -
KW560_RS29445 6519..6842 + 324 WP_085842301.1 hypothetical protein -
KW560_RS28790 (KW560_28765) 6883..7299 - 417 WP_048983057.1 type II toxin-antitoxin system VapC family toxin Toxin
KW560_RS28795 (KW560_28770) 7296..7526 - 231 WP_016338373.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KW560_RS28800 (KW560_28775) 8091..8309 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
KW560_RS28805 (KW560_28780) 8311..8616 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
KW560_RS28810 (KW560_28785) 8617..9423 + 807 WP_000016982.1 site-specific integrase -
KW560_RS28815 (KW560_28790) 10197..10952 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..29628 29628


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15022.52 Da        Isoelectric Point: 9.2957

>T209656 WP_048983057.1 NZ_CP079658:c7299-6883 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVK

Download         Length: 417 bp

>T209656 NZ_CP104206:c2836960-2836706 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8751.77 Da        Isoelectric Point: 4.3533

>AT209656 WP_016338373.1 NZ_CP079658:c7526-7296 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWSSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT209656 NZ_CP104206:c2837208-2836957 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNG2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q9LN61

References