Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5415565..5416190 Replicon chromosome
Accession NZ_CP079655
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC27_S471MPC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KW560_RS26570 Protein ID WP_002882817.1
Coordinates 5415565..5415948 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KW560_RS26575 Protein ID WP_004150355.1
Coordinates 5415948..5416190 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW560_RS26555 (5412931) 5412931..5413833 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KW560_RS26560 (5413830) 5413830..5414465 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KW560_RS26565 (5414462) 5414462..5415391 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
KW560_RS26570 (5415565) 5415565..5415948 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KW560_RS26575 (5415948) 5415948..5416190 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KW560_RS26580 (5416395) 5416395..5417312 + 918 WP_004181612.1 alpha/beta hydrolase -
KW560_RS26585 (5417326) 5417326..5418267 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KW560_RS26590 (5418312) 5418312..5418749 - 438 WP_064162494.1 D-aminoacyl-tRNA deacylase -
KW560_RS26595 (5418746) 5418746..5419606 - 861 WP_004146232.1 virulence factor BrkB family protein -
KW560_RS26600 (5419600) 5419600..5420199 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209654 WP_002882817.1 NZ_CP079655:c5415948-5415565 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209654 NZ_CP104206:c2362572-2362285 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209654 WP_004150355.1 NZ_CP079655:c5416190-5415948 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209654 NZ_CP104206:c2362811-2362572 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References