Detailed information of TA system TA209649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2773645..2775233
• Replicon: chromosome
• Accession: NZ_CP079655
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC27_S471MPC

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KW560_RS13725
• Protein ID: WP_064162061.1
• Coordinates: 2773645..2774967 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: KW560_RS13730
• Protein ID: WP_002904790.1
• Coordinates: 2774967..2775233 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW560_RS13710 (2768701)	2768701..2771202	+	2502	WP_064162059.1	fimbria/pilus outer membrane usher protein	-
KW560_RS13715 (2771190)	2771190..2772194	+	1005	WP_064162060.1	fimbrial protein	-
KW560_RS13720 (2772336)	2772336..2773628	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
KW560_RS13725 (2773645)	2773645..2774967	-	1323	WP_064162061.1	type II toxin-antitoxin system HipA family toxin	Toxin
KW560_RS13730 (2774967)	2774967..2775233	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KW560_RS13735 (2775499)	2775499..2775825	-	327	WP_064162062.1	hydroxyisourate hydrolase	-
KW560_RS13740 (2775822)	2775822..2776322	-	501	WP_064162063.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KW560_RS13745 (2776300)	2776300..2777679	-	1380	WP_040245186.1	nucleobase:cation symporter-2 family protein	-
KW560_RS13750 (2778025)	2778025..2779179	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
KW560_RS13755 (2779160)	2779160..2780083	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49155.46 Da
• Isoelectric Point: 7.5357

>T209649 WP_064162061.1 NZ_CP079655:c2774967-2773645 [Klebsiella pneumoniae subsp. pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T209649 NZ_CP104206:c2016800-2016693 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT209649 WP_002904790.1 NZ_CP079655:c2775233-2774967 [Klebsiella pneumoniae subsp. pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT209649 NZ_CP104206:2016853-2016914 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8