Detailed information of TA system TA020964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-RelB
• Location: 1474137..1474660
• Replicon: chromosome
• Accession: NC_004757
• Organism: Nitrosomonas europaea ATCC 19718

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ALW85_RS07020
• Protein ID: WP_011111923.1
• Coordinates: 1474137..1474397 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: ALW85_RS07025
• Protein ID: WP_041356746.1
• Coordinates: 1474400..1474660 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ALW85_RS06985 (NE1339)	1469525..1469815	-	291	WP_011111917.1	H-NS histone family protein	-
ALW85_RS06990 (NE1340)	1469837..1470079	-	243	WP_011111918.1	hypothetical protein	-
ALW85_RS06995 (NE1341)	1470125..1470676	-	552	WP_041356742.1	hypothetical protein	-
ALW85_RS07000 (NE1342)	1470676..1471698	-	1023	WP_143023681.1	glycosyl transferase	-
ALW85_RS07005 (NE1343)	1471770..1472942	-	1173	WP_011111921.1	nucleotide sugar dehydrogenase	-
ALW85_RS07010 (RK28_RS07005)	1473150..1473380	-	231	WP_041356743.1	dTDP-4-dehydrorhamnose 3,5-epimerase family protein	-
ALW85_RS14185 (NE1344)	1473509..1473787	-	279	WP_011111922.1	hypothetical protein	-
ALW85_RS07020 (NE1345)	1474137..1474397	-	261	WP_011111923.1	Txe/YoeB family addiction module toxin	Toxin
ALW85_RS07025 (NE1346)	1474400..1474660	-	261	WP_041356746.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ALW85_RS07030 (NE1347)	1474936..1475978	+	1043	Protein_1357	IS481 family transposase	-
ALW85_RS07035 (NE1349)	1476105..1476500	-	396	WP_011111927.1	type II toxin-antitoxin system VapC family toxin	-
ALW85_RS07040 (NE1350)	1476508..1476753	-	246	WP_011111928.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
ALW85_RS07050 (NE1351)	1477435..1478478	+	1044	WP_011110809.1	IS481 family transposase	-
ALW85_RS07055 (NE1352)	1478535..1478795	-	261	WP_011111929.1	Txe/YoeB family addiction module toxin	-
ALW85_RS07060 (RK28_RS07055)	1478795..1479037	-	243	WP_041356748.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ALW85_RS07065 (RK28_RS07060)	1479355..1479621	+	267	WP_041357169.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-75)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10200.66 Da
• Isoelectric Point: 8.8925

>T20964 WP_011111923.1 NC_004757:c1474397-1474137 [Nitrosomonas europaea ATCC 19718]
VRAIRFVPDAWEAYLYWQDQDKKTLRRLNSLITAASRDPFVGIGKPEPLRGELSGYWSRRIDETNRLVYRVTDVELVIIA
CRFHYE

Nucleotide

• Length: 261 bp

>T20964 NC_004757:c1474397-1474137 [Nitrosomonas europaea ATCC 19718]
GTGCGTGCCATTCGTTTTGTTCCTGATGCGTGGGAGGCTTACCTTTACTGGCAAGACCAGGATAAAAAAACGCTCAGGCG
ATTGAATTCTCTGATTACTGCCGCTTCTCGTGATCCATTTGTTGGTATTGGCAAACCAGAACCACTGCGGGGTGAATTGT
CGGGTTATTGGTCAAGACGTATCGATGAAACTAATCGTTTGGTTTATCGTGTTACTGATGTTGAGTTAGTGATTATTGCT
TGCCGATTTCACTATGAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9161.30 Da
• Isoelectric Point: 4.6470

>AT20964 WP_041356746.1 NC_004757:c1474660-1474400 [Nitrosomonas europaea ATCC 19718]
MKVVTYSHARNALKSILDDVIQDADVIVISRRDAEGDAVVMSLDSYNSIMETLHLTSNPANAAALAKAIAQDKAGQAQDH
PLLSAD

Nucleotide

• Length: 261 bp

>AT20964 NC_004757:c1474660-1474400 [Nitrosomonas europaea ATCC 19718]
ATGAAGGTTGTTACTTATTCGCATGCGCGTAATGCGTTAAAGTCTATTTTGGATGATGTCATTCAGGATGCTGATGTAAT
TGTTATTAGTCGTCGCGATGCAGAAGGTGATGCTGTGGTGATGTCGCTGGATAGCTATAACAGCATCATGGAAACATTGC
ACTTAACCAGTAATCCAGCAAATGCCGCAGCCTTAGCCAAGGCAATTGCTCAGGATAAGGCAGGACAAGCACAAGACCAC
CCATTGCTTTCTGCCGATTAA