Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 111090..111615 Replicon plasmid pS468M_P1
Accession NZ_CP079646
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC24_S468MC

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag KW559_RS27630 Protein ID WP_001159868.1
Coordinates 111310..111615 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag KW559_RS27625 Protein ID WP_000813634.1
Coordinates 111090..111308 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW559_RS27605 (KW559_27580) 107028..108305 - 1278 WP_016338369.1 HlyD family secretion protein -
KW559_RS28315 108964..109500 + 537 WP_223825639.1 hypothetical protein -
KW559_RS28320 109518..109841 + 324 WP_085842301.1 hypothetical protein -
KW559_RS27615 (KW559_27590) 109882..110298 - 417 WP_048983057.1 type II toxin-antitoxin system VapC family toxin -
KW559_RS27620 (KW559_27595) 110295..110525 - 231 WP_016338373.1 type II toxin-antitoxin system VapB family antitoxin -
KW559_RS27625 (KW559_27600) 111090..111308 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KW559_RS27630 (KW559_27605) 111310..111615 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
KW559_RS27635 (KW559_27610) 111616..112422 + 807 WP_000016982.1 site-specific integrase -
KW559_RS27640 (KW559_27615) 113196..113951 + 756 WP_000852146.1 replication initiation protein RepE -
KW559_RS27645 (KW559_27620) 114539..115705 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCMY-4 - 1..150711 150711


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T209638 WP_001159868.1 NZ_CP079646:111310-111615 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T209638 NZ_CP104199:c469-188 [Klebsiella pneumoniae]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT209638 WP_000813634.1 NZ_CP079646:111090-111308 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT209638 NZ_CP104199:c710-459 [Klebsiella pneumoniae]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References