Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 109882..110525 Replicon plasmid pS468M_P1
Accession NZ_CP079646
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC24_S468MC

Toxin (Protein)


Gene name vagD Uniprot ID A0A5Q9LNG2
Locus tag KW559_RS27615 Protein ID WP_048983057.1
Coordinates 109882..110298 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID R4IT22
Locus tag KW559_RS27620 Protein ID WP_016338373.1
Coordinates 110295..110525 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW559_RS27600 (KW559_27575) 104930..107038 - 2109 WP_016338368.1 peptidase domain-containing ABC transporter -
KW559_RS27605 (KW559_27580) 107028..108305 - 1278 WP_016338369.1 HlyD family secretion protein -
KW559_RS28315 108964..109500 + 537 WP_223825639.1 hypothetical protein -
KW559_RS28320 109518..109841 + 324 WP_085842301.1 hypothetical protein -
KW559_RS27615 (KW559_27590) 109882..110298 - 417 WP_048983057.1 type II toxin-antitoxin system VapC family toxin Toxin
KW559_RS27620 (KW559_27595) 110295..110525 - 231 WP_016338373.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KW559_RS27625 (KW559_27600) 111090..111308 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
KW559_RS27630 (KW559_27605) 111310..111615 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
KW559_RS27635 (KW559_27610) 111616..112422 + 807 WP_000016982.1 site-specific integrase -
KW559_RS27640 (KW559_27615) 113196..113951 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCMY-4 - 1..150711 150711


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15022.52 Da        Isoelectric Point: 9.2957

>T209637 WP_048983057.1 NZ_CP079646:c110298-109882 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVK

Download         Length: 417 bp

>T209637 NZ_CP104198:c31556-31140 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8751.77 Da        Isoelectric Point: 4.3533

>AT209637 WP_016338373.1 NZ_CP079646:c110525-110295 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWSSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT209637 NZ_CP104198:c31783-31553 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNG2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q9LN61

References