Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5416344..5416969 Replicon chromosome
Accession NZ_CP079645
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC24_S468MC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KW559_RS26575 Protein ID WP_002882817.1
Coordinates 5416344..5416727 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KW559_RS26580 Protein ID WP_004150355.1
Coordinates 5416727..5416969 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW559_RS26560 (5413710) 5413710..5414612 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KW559_RS26565 (5414609) 5414609..5415244 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KW559_RS26570 (5415241) 5415241..5416170 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
KW559_RS26575 (5416344) 5416344..5416727 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KW559_RS26580 (5416727) 5416727..5416969 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KW559_RS26585 (5417174) 5417174..5418091 + 918 WP_004181612.1 alpha/beta hydrolase -
KW559_RS26590 (5418105) 5418105..5419046 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KW559_RS26595 (5419091) 5419091..5419528 - 438 WP_064162494.1 D-aminoacyl-tRNA deacylase -
KW559_RS26600 (5419525) 5419525..5420385 - 861 WP_004146232.1 virulence factor BrkB family protein -
KW559_RS26605 (5420379) 5420379..5420978 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209635 WP_002882817.1 NZ_CP079645:c5416727-5416344 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209635 NZ_CP104196:c5260964-5260581 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209635 WP_004150355.1 NZ_CP079645:c5416969-5416727 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209635 NZ_CP104196:c5261206-5260964 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References