Detailed information of TA system TA020962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1426777..1427324
• Replicon: chromosome
• Accession: NC_004757
• Organism: Nitrosomonas europaea ATCC 19718

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ALW85_RS06795
• Protein ID: WP_011111884.1
• Coordinates: 1426777..1427103 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A837WUI4
• Locus tag: ALW85_RS06800
• Protein ID: WP_041356736.1
• Coordinates: 1427100..1427324 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ALW85_RS06765 (NE1300)	1423064..1423339	-	276	WP_011111878.1	hypothetical protein	-
ALW85_RS06770 (NE1301)	1423652..1424122	+	471	WP_011111879.1	isoprenylcysteine carboxylmethyltransferase family protein	-
ALW85_RS06775 (NE1302)	1424274..1425341	-	1068	WP_041356735.1	Rpn family recombination-promoting nuclease/putative transposase	-
ALW85_RS06780 (NE1303)	1425501..1425992	+	492	WP_011111881.1	VCBS repeat-containing protein	-
ALW85_RS06785 (NE1304)	1426056..1426358	-	303	WP_011111882.1	HigA family addiction module antidote protein	-
ALW85_RS06790 (NE1305)	1426371..1426649	-	279	WP_011111883.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ALW85_RS06795 (NE1306)	1426777..1427103	-	327	WP_011111884.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ALW85_RS06800 (NE1307)	1427100..1427324	-	225	WP_041356736.1	antitoxin MazE family protein	Antitoxin
ALW85_RS06805 (NE1308)	1427752..1428162	-	411	WP_011111886.1	pilin	-
ALW85_RS13630 (NE1309)	1428787..1429083	-	297	WP_011111887.1	DUF2335 domain-containing protein	-
ALW85_RS06810 (NE1310)	1429522..1430811	-	1290	WP_011111888.1	type II toxin-antitoxin system HipA family toxin	-
ALW85_RS06815 (NE1311)	1430801..1431061	-	261	WP_041356737.1	helix-turn-helix transcriptional regulator	-
ALW85_RS06825 (NE1312)	1431744..1431947	-	204	WP_011111890.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (8-72)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11481.69 Da
• Isoelectric Point: 6.9477

>T20962 WP_011111884.1 NC_004757:c1427103-1426777 [Nitrosomonas europaea ATCC 19718]
MIRGDLVTIAVPGDFGKPGFALVIQANLFSEHTSVTVLPVTSMLVAAPLLRITVQPGAENGLQKPSQVMVDKIITVKRDK
VGPVLGCIDPDTMVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T20962 NC_004757:c1427103-1426777 [Nitrosomonas europaea ATCC 19718]
ATGATACGCGGCGATCTCGTGACCATCGCTGTACCGGGCGATTTTGGTAAACCCGGATTCGCTCTGGTGATTCAGGCTAA
CCTGTTCAGTGAACATACCAGCGTAACGGTATTACCTGTCACCAGCATGCTGGTTGCTGCGCCATTGCTACGTATCACTG
TACAGCCTGGTGCCGAGAACGGTTTGCAAAAACCTTCGCAGGTAATGGTGGACAAGATCATAACGGTGAAACGCGACAAA
GTAGGGCCGGTTCTCGGATGTATCGACCCGGATACTATGGTAGAGATTGAGCGTTGCCTGGCTGTGTTCCTTGGAATAGC
GAAATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8516.64 Da
• Isoelectric Point: 6.4497

>AT20962 WP_041356736.1 NC_004757:c1427324-1427100 [Nitrosomonas europaea ATCC 19718]
MGTTHVNARVKKHRDTLRMAGLRPVQIWVPDTRRPDFAEECRRQCLLIAQADKADTSMQQFMDEALADSDGWTE

Nucleotide

• Length: 225 bp

>AT20962 NC_004757:c1427324-1427100 [Nitrosomonas europaea ATCC 19718]
ATGGGAACCACGCATGTTAATGCTCGTGTTAAAAAGCACCGTGACACACTACGCATGGCCGGATTACGTCCGGTACAAAT
CTGGGTACCGGACACCCGCCGTCCGGATTTTGCAGAAGAATGCCGCCGCCAGTGCCTTCTCATAGCTCAGGCGGACAAAG
CGGATACATCCATGCAACAGTTCATGGATGAAGCTTTGGCCGATAGTGATGGCTGGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A837WUI4