Detailed information of TA system TA209619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 55117..55642
• Replicon: plasmid pAI1646M_P2
• Accession: NZ_CP079644
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC22_AI1646MC

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: KW558_RS27660
• Protein ID: WP_001159868.1
• Coordinates: 55337..55642 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: KW558_RS27655
• Protein ID: WP_000813634.1
• Coordinates: 55117..55335 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW558_RS27625 (KW558_27590)	50510..50926	-	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	-
KW558_RS27630 (KW558_27595)	50923..51153	-	231	WP_001261278.1	type II toxin-antitoxin system VapB family antitoxin	-
KW558_RS27635 (KW558_27600)	51418..51918	+	501	WP_000528931.1	HEPN family nuclease	-
KW558_RS27640 (KW558_27605)	51931..52704	+	774	WP_000905949.1	hypothetical protein	-
KW558_RS27645 (KW558_27610)	52871..54004	+	1134	WP_000545987.1	DUF3800 domain-containing protein	-
KW558_RS27650 (KW558_27615)	54038..54526	-	489	WP_011254646.1	hypothetical protein	-
KW558_RS27655 (KW558_27620)	55117..55335	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KW558_RS27660 (KW558_27625)	55337..55642	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
KW558_RS27665 (KW558_27630)	55643..56449	+	807	WP_000016982.1	site-specific integrase	-
KW558_RS27670 (KW558_27635)	57223..57978	+	756	WP_000852146.1	replication initiation protein RepE	-
KW558_RS27675 (KW558_27640)	58566..59732	+	1167	WP_000772446.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / sitABCD	iucA / iucB / iucC / iucD / iutA	1..73277	73277

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T209619 WP_001159868.1 NZ_CP079644:55337-55642 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T209619 NZ_CP104172:6506968-6507258 [Bradyrhizobium sp. CB3035]
ATGGTCGAGGTGAGGCAGACTGAGCATTTCTCGGAGTGGCTGAGCCGCCTCAGGGACGCCAGCGCGGTAGCCAGGATCAC
CGTCCGGATTCGCCGCATGGAGATGGGCAATCCCGGTGACAGCAAGAGCGTCGGCCGCAACATCCGGGAAATGCGCATCG
ATTACGGCCCGGGCTACCGGATTTACTATGTACAGCGCGGCGCGCAGATCGTGATCCTGCTGTGTGGCGGCGACAAGCGC
ACACAACGACAAGACATCGAGCTAGCCCAGCAACTGGCGGAGACATCGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT209619 WP_000813634.1 NZ_CP079644:55117-55335 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT209619 NZ_CP104172:6507258-6507608 [Bradyrhizobium sp. CB3035]
ATGCCAAAGGCAGCAAAAACGACGGCCAAAACGGCATCCAAGACGACGCGTTTCGACGCCGCCGACTATCTCAACACCGA
AGAGCGTCAGGCCGCCTATATCGCAGCGGCCCTCGAAACCGGCGACGCCGATTTCGTGCGTGACGCGCTTGGCCTCGTCG
CGCGTGCGCGCGGCATGAGTGCGATCGCGAAGAAAGCCGGCTTGAACCGCGAGAGCCTGTACAAGGCCCTCGGAGAGACC
GGAAATCCCGAGTTCGGCACGGTCATGCGCATTGTCGGAGCGTTGGGTCTGACACTATCAGCCCAACCCGCGACAAGCGG
ACGAACGCCAAAGCGCCGTCGCGCCGCTTGA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2