Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 50510..51153 Replicon plasmid pAI1646M_P2
Accession NZ_CP079644
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC22_AI1646MC

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag KW558_RS27625 Protein ID WP_001034046.1
Coordinates 50510..50926 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag KW558_RS27630 Protein ID WP_001261278.1
Coordinates 50923..51153 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW558_RS27610 (KW558_27575) 45647..46063 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
KW558_RS27615 (KW558_27580) 46060..46290 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
KW558_RS27620 (KW558_27585) 46671..50465 + 3795 WP_001144732.1 hypothetical protein -
KW558_RS27625 (KW558_27590) 50510..50926 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
KW558_RS27630 (KW558_27595) 50923..51153 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KW558_RS27635 (KW558_27600) 51418..51918 + 501 WP_000528931.1 HEPN family nuclease -
KW558_RS27640 (KW558_27605) 51931..52704 + 774 WP_000905949.1 hypothetical protein -
KW558_RS27645 (KW558_27610) 52871..54004 + 1134 WP_000545987.1 DUF3800 domain-containing protein -
KW558_RS27650 (KW558_27615) 54038..54526 - 489 WP_011254646.1 hypothetical protein -
KW558_RS27655 (KW558_27620) 55117..55335 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
KW558_RS27660 (KW558_27625) 55337..55642 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / sitABCD iucA / iucB / iucC / iucD / iutA 1..73277 73277


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T209618 WP_001034046.1 NZ_CP079644:c50926-50510 [Klebsiella pneumoniae subsp. pneumoniae]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T209618 NZ_CP104171:c6947578-6947390 [Bradyrhizobium sp. NC92]
ATGAAGTCGACGGACATCATTGCAGCCCTAAAAGCCGACGGTTGGACGCAGGTGGCCCAGAAAGGCAGCCACGTTCAGTT
CAAGCATCCGATCAAACCGGGGCGCGTGACGGTCCCTCACCCTAAGAAGGACATTCCGATTGGAACGGTCCGGAGCATCG
AAAAGCAGTCCGTACTGAAGCTGAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT209618 WP_001261278.1 NZ_CP079644:c51153-50923 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT209618 NZ_CP104171:c6947337-6946945 [Bradyrhizobium sp. NC92]
ATGCGATACTACATCGTCCTAATTCACAAGGACGCAGATAGCGACTACGGCGTCTCGTTTCCTGATCTTCCTGGCGTCAT
CAGCGCAGGAAGCACTTTGGACGAAGCTCGCGAGATGGCGGCGGAAGCTTTGGCCCTTCATCTTCGAGGATTGGCTGAGG
ATGGTGAAGCCGTTCCAGAGCCTTCGTCTCTCGAGCAGATCATGGCCAATGCAGATAACAAGGATGGCGTTGCCGTCTTG
ATCCCGGCCCCAGCCGAAGAGGTAAAGAGCGTGCGCGTGAACATCACCCTGCCCGCGGACGTTCTGAGCGAGATTGACCG
ACACGCGGAGCAGCAAGGCTTCACGCGTTCTGGCTTCCTTGCTCAGGCAGCGAAGAAGGCGTTGGCAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References