Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5362068..5362693 Replicon chromosome
Accession NZ_CP079642
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC22_AI1646MC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KW558_RS26270 Protein ID WP_002882817.1
Coordinates 5362068..5362451 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KW558_RS26275 Protein ID WP_004150355.1
Coordinates 5362451..5362693 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW558_RS26255 (5359434) 5359434..5360336 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KW558_RS26260 (5360333) 5360333..5360968 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KW558_RS26265 (5360965) 5360965..5361894 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
KW558_RS26270 (5362068) 5362068..5362451 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KW558_RS26275 (5362451) 5362451..5362693 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KW558_RS26280 (5362898) 5362898..5363815 + 918 WP_004181612.1 alpha/beta hydrolase -
KW558_RS26285 (5363829) 5363829..5364770 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KW558_RS26290 (5364815) 5364815..5365252 - 438 WP_064162494.1 D-aminoacyl-tRNA deacylase -
KW558_RS26295 (5365249) 5365249..5366109 - 861 WP_004146232.1 virulence factor BrkB family protein -
KW558_RS26300 (5366103) 5366103..5366702 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209616 WP_002882817.1 NZ_CP079642:c5362451-5362068 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209616 NZ_CP104170:c4871977-4871699 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209616 WP_004150355.1 NZ_CP079642:c5362693-5362451 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209616 NZ_CP104170:c4871687-4871382 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCCGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References