Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4740039..4740849 Replicon chromosome
Accession NZ_CP079636
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC21_AI1646PC

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KW557_RS23365 Protein ID WP_004178461.1
Coordinates 4740039..4740572 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KW557_RS23370 Protein ID WP_002887278.1
Coordinates 4740583..4740849 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW557_RS23360 (4738870) 4738870..4739991 + 1122 WP_004214138.1 cupin domain-containing protein -
KW557_RS23365 (4740039) 4740039..4740572 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KW557_RS23370 (4740583) 4740583..4740849 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KW557_RS23375 (4740952) 4740952..4742385 - 1434 WP_064162551.1 Cu(+)/Ag(+) sensor histidine kinase -
KW557_RS23380 (4742375) 4742375..4743058 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KW557_RS23385 (4743230) 4743230..4744615 + 1386 WP_064162550.1 efflux transporter outer membrane subunit -
KW557_RS23390 (4744633) 4744633..4744977 + 345 WP_064162549.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T209595 WP_004178461.1 NZ_CP079636:c4740572-4740039 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T209595 NZ_CP104149:c354801-354526 [Sinorhizobium medicae]
ATGAAGCTTGAGTGGACGAGTAAGGCAGTCGCCGATCTTGGGCGCCTCTATGATTTCCTGGCACCGGTAAATCGGCAAGC
TGCGGCTCGTACTGTGCAATCGTTGACGAGCGCTCCAGCACGCCTGCTCGAACAACCGCGTATCGGCGAACGGCTGGAGG
AATTCGACCCTCGAGAGGTTCGGCGAATTCTCGTTGGCCATTACGAAATACGGTATGAGATCCGGCAGTCGACGATCTAC
GTGCTGCGGCTTTGGCATACGCGTGAAGACCGTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT209595 WP_002887278.1 NZ_CP079636:c4740849-4740583 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT209595 NZ_CP104149:c355061-354801 [Sinorhizobium medicae]
ATGGAAACGAAGGTGCTGACGGCGCACGTACCTCTGCCGCTCGCCGAGAAAGTCGATCAGATCGCGGCCCGGCTTGAGCG
CTCGCGCGGGTGGATCGTCAAGCAGGCGCTGACCGCTTGGGTCGATCAGGAAGAGGAGCGCCGACGCTTGACGCTGGAAG
CGCTTGCCGACGTCGACGCTGACCGCGTTATCGATCACCAGGCCGTCCAGGCCTGGGCCGACAGTCTTGATAGTGACAAG
CCGCTATCTTTGCCGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References