Detailed information of TA system TA020959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1195700..1196463
• Replicon: chromosome
• Accession: NC_004757
• Organism: Nitrosomonas europaea ATCC 19718

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q82VI1
• Locus tag: ALW85_RS05730
• Protein ID: WP_011111700.1
• Coordinates: 1195700..1196194 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q82VI0
• Locus tag: ALW85_RS05735
• Protein ID: WP_011111701.1
• Coordinates: 1196191..1196463 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ALW85_RS05710 (NE1098)	1192020..1193006	-	987	WP_011111695.1	FecR family protein	-
ALW85_RS05715 (NE1099)	1193020..1193523	-	504	WP_011111696.1	sigma-70 family RNA polymerase sigma factor	-
ALW85_RS05720 (NE1101)	1194094..1194585	+	492	WP_198407871.1	sigma-70 family RNA polymerase sigma factor	-
ALW85_RS05725 (NE1102)	1194736..1195668	+	933	WP_198407872.1	FecR domain-containing protein	-
ALW85_RS05730 (NE1103)	1195700..1196194	-	495	WP_011111700.1	GNAT family N-acetyltransferase	Toxin
ALW85_RS05735 (NE1104)	1196191..1196463	-	273	WP_011111701.1	DUF1778 domain-containing protein	Antitoxin
ALW85_RS05740 (NE1105)	1196708..1198576	+	1869	WP_050731249.1	TonB-dependent receptor	-
ALW85_RS13940	1198601..1199376	+	776	Protein_1113	IS5 family transposase	-
ALW85_RS05755 (NE1108)	1199381..1200004	+	624	Protein_1114	TonB-dependent receptor	-
ALW85_RS05760 (NE1109)	1200249..1200530	+	282	WP_011111702.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ALW85_RS05765 (NE1110)	1200573..1200899	+	327	WP_050731300.1	HigA family addiction module antidote protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1199008..1199376	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (28-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18024.67 Da
• Isoelectric Point: 9.4426

>T20959 WP_011111700.1 NC_004757:c1196194-1195700 [Nitrosomonas europaea ATCC 19718]
MSLQLNPPELLVATHLLDDFECGVNSLDEWLKRRALANQHSGASRTFVVADHDSRVYGYYAMAAGAVSHQAATSGVRRNM
PDPVPVMVLARLAVDQRAQGIKLGAALLQDAVNRVVNVSHNVGVRALLVHALDDRAKQFYAHYGFKESPQHPMTLMLRLN
TTKA

Nucleotide

• Length: 495 bp

>T20959 NC_004757:c1196194-1195700 [Nitrosomonas europaea ATCC 19718]
ATGAGCTTGCAACTCAATCCGCCAGAGCTGCTCGTCGCAACGCATCTTCTGGACGACTTCGAGTGCGGAGTAAACAGTCT
CGATGAATGGCTCAAGCGCCGGGCATTAGCCAACCAGCATAGCGGAGCCAGCCGTACATTCGTTGTCGCGGATCACGATA
GTCGCGTCTATGGTTACTACGCCATGGCTGCGGGTGCAGTCTCGCATCAAGCGGCAACGAGCGGTGTGCGGCGCAACATG
CCCGATCCTGTTCCGGTGATGGTGTTAGCACGACTGGCCGTTGATCAGCGTGCCCAAGGTATCAAACTCGGGGCAGCATT
GCTGCAAGATGCCGTCAATCGAGTGGTAAACGTTTCGCACAATGTTGGTGTCCGAGCGCTTCTCGTCCATGCGCTCGATG
ACCGTGCCAAGCAGTTCTACGCGCACTACGGTTTCAAGGAATCACCCCAGCATCCAATGACTTTAATGCTGCGCCTAAAC
ACGACAAAGGCATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10003.46 Da
• Isoelectric Point: 4.4948

>AT20959 WP_011111701.1 NC_004757:c1196463-1196191 [Nitrosomonas europaea ATCC 19718]
MRDATINLRALPEQRDLIDQAASLLGKNRSDFMLEAACDRAQAVVLDQVFFSLDTDKFKQFTTILDAPPGPNPGLERLMA
VKAPWNTDVV

Nucleotide

• Length: 273 bp

>AT20959 NC_004757:c1196463-1196191 [Nitrosomonas europaea ATCC 19718]
ATGCGTGACGCTACCATTAACCTGCGTGCCCTGCCCGAACAACGCGACCTTATTGATCAGGCCGCCAGTTTGCTCGGCAA
AAACCGTTCGGACTTCATGCTCGAAGCAGCGTGCGACCGAGCACAGGCCGTTGTGTTGGATCAGGTCTTTTTTAGCCTGG
ACACCGACAAGTTTAAGCAGTTTACTACCATACTCGATGCACCTCCCGGCCCCAACCCAGGGCTTGAACGCCTTATGGCC
GTGAAAGCTCCCTGGAATACCGATGTGGTATGA

Structures

Toxin

• AlphaFold DB: Q82VI1

Antitoxin

• AlphaFold DB: Q82VI0