Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2621136..2621761 Replicon chromosome
Accession NZ_CP079634
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC19_AI1572C

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KW555_RS12720 Protein ID WP_002882817.1
Coordinates 2621136..2621519 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KW555_RS12725 Protein ID WP_004150355.1
Coordinates 2621519..2621761 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW555_RS12705 (2618502) 2618502..2619404 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KW555_RS12710 (2619401) 2619401..2620036 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KW555_RS12715 (2620033) 2620033..2620962 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
KW555_RS12720 (2621136) 2621136..2621519 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KW555_RS12725 (2621519) 2621519..2621761 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KW555_RS12730 (2621966) 2621966..2622883 + 918 WP_004181612.1 alpha/beta hydrolase -
KW555_RS12735 (2622897) 2622897..2623838 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KW555_RS12740 (2623883) 2623883..2624320 - 438 WP_064162494.1 D-aminoacyl-tRNA deacylase -
KW555_RS12745 (2624317) 2624317..2625177 - 861 WP_004146232.1 virulence factor BrkB family protein -
KW555_RS12750 (2625171) 2625171..2625770 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209570 WP_002882817.1 NZ_CP079634:c2621519-2621136 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209570 NZ_CP104136:563361-563885 [Sinorhizobium sp. C101]
GTGCTTGACTGGCGGGAGGAACCTATCGGGCGCCACCATGACCGCAAGGCGTTCGATTGCGGCACGCCGGAACTGAACGA
GTATCTACGCCGGCACGCCCGTCAGAACCACGAAGGCGGCGGCTCCAAGACCTTCGTGGCGGTGGCTCCAACCGCGCCCG
AAACCATACTCGGCTACTATTCCATCAGCCCCGCTTCAATTGCCTTCGCAAAAGTCCCCGCCCCCCTGACCAAAGGATTG
GGCCGCTACGAGGTCGCCGTGTATCGGCTTGCCCGTCTGGCGGTGGCGCTTCCGCTGCAGGGTCGGGGGCTTGGCGGCGA
TCTTTTGCTGGCAGCCGGTGCGCGAGCGCTTGCCGTTGCCGCGCAGGTCGGCGGGGTGGCGCTTGCCATCGACGCCAAGG
ACGAGAAGGCGGCGTGCTGGTACGAGCGTTTCGGCGCAATGCCGCTGCTCGACAGCAGCCCCCTTAGCCTGATCCTGCCA
TTCAAGACCATCATCGACGCTCTGGATGCCGCGCAGGGCGAATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209570 WP_004150355.1 NZ_CP079634:c2621761-2621519 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209570 NZ_CP104136:563078-563368 [Sinorhizobium sp. C101]
ATGGCCCAGATGCAAGCTAATCGTGACGACCGCATCGAACTGCGCACCACGCGCGACGAAAAGCGGCTTCTCACCGCCGC
CGCCGCCCATGAGCGGCTGGACGTAACCAGCTTCATCATGCGCGCTGTCCTGCCGGCCGCGCGCGAGGTGGTTGAGAACG
CGGAACGTATTTCGCTGTCGGAGCGGGATTCCCTGCGCGTGCTCGATCTTCTGGAAAATCCGCCGGCCCCGACGCCGGCT
CTGCTCGCCGCAGCCCGCCGCCGTATCGCACGAGACGGCAAGAGTGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References