Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 32743..33386 Replicon plasmid pCMC307M_P3
Accession NZ_CP079632
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC18_CMC307MC

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KW554_RS29270 Protein ID WP_001044770.1
Coordinates 32970..33386 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KW554_RS29265 Protein ID WP_001261282.1
Coordinates 32743..32973 (+) Length 77 a.a.

Genomic Context


KW554_RS29235KW554_RS29240KW554_RS29265KW554_RS29270KW554_RS29275KW554_RS29280KW554_RS29230KW554_RS29245KW554_RS29250KW554_RS29255KW554_RS29260KW554_RS29285KW554_RS29290KW554_RS29295KW554_RS29300
Location: 29716..29820 (105 bp)
Type: Others
Protein ID: WP_032409716.1
Location: 29949..30206 (258 bp)
Type: Others
Protein ID: WP_000764642.1
Location: 32743..32973 (231 bp)
Type: Antitoxin
Protein ID: WP_001261282.1
Location: 32970..33386 (417 bp)
Type: Toxin
Protein ID: WP_001044770.1
Location: 33460..34149 (690 bp)
Type: Others
Protein ID: Protein_45
Location: 34204..34901 (698 bp)
Type: Others
Protein ID: WP_223174630.1
Location: 28315..29181 (867 bp)
Type: Others
Protein ID: WP_004118283.1
Location: 30264..31040 (777 bp)
Type: Others
Protein ID: WP_000015958.1
Location: 31037..31780 (744 bp)
Type: Others
Protein ID: WP_000129823.1
Location: 31831..32181 (351 bp)
Type: Others
Protein ID: WP_000493378.1
Location: 32325..32786 (462 bp)
Type: Others
Protein ID: WP_072202616.1
Location: 34986..35846 (861 bp)
Type: Others
Protein ID: WP_000027057.1
Location: 36029..36346 (318 bp)
Type: Others
Protein ID: Protein_48
Location: 36546..37370 (825 bp)
Type: Others
Protein ID: WP_000722315.1
Location: 37430..38218 (789 bp)
Type: Others
Protein ID: WP_001206315.1
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
KW554_RS29230 (KW554_29215)28315..29181-867WP_004118283.1replication initiation protein-
KW554_RS29235 (KW554_29220)29716..29820+105WP_032409716.1hypothetical protein-
KW554_RS29240 (KW554_29225)29949..30206+258WP_000764642.1hypothetical protein-
KW554_RS29245 (KW554_29230)30264..31040-777WP_000015958.1site-specific integrase-
KW554_RS29250 (KW554_29235)31037..31780-744WP_000129823.1hypothetical protein-
KW554_RS29255 (KW554_29240)31831..32181-351WP_000493378.1hypothetical protein-
KW554_RS29260 (KW554_29245)32325..32786-462WP_072202616.1hypothetical protein-
KW554_RS29265 (KW554_29250)32743..32973+231WP_001261282.1type II toxin-antitoxin system VapB family antitoxinAntitoxin
KW554_RS29270 (KW554_29255)32970..33386+417WP_001044770.1type II toxin-antitoxin system VapC family toxinToxin
KW554_RS29275 (KW554_29260)33460..34149+690Protein_45AAA family ATPase-
KW554_RS29280 (KW554_29265)34204..34901+698WP_223174630.1IS1 family transposase-
KW554_RS29285 (KW554_29270)34986..35846-861WP_000027057.1broad-spectrum class A beta-lactamase TEM-1-
KW554_RS29290 (KW554_29275)36029..36346-318Protein_48recombinase family protein-
KW554_RS29295 (KW554_29280)36546..37370-825WP_000722315.1oxacillin-hydrolyzing class D beta-lactamase OXA-9-
KW554_RS29300 (KW554_29285)37430..38218-789WP_001206315.1ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1-

Associated MGEs


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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
-insideMobilizable plasmidaph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaCTX-M-15 / aac(3)-IId / blaSHV-187 / ARR-3 / ere(A) / cmlA1 / qacE / sul1 / blaOXA-181-1..7712877128


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-129)

Antitoxin

(7-47)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T209565 WP_001044770.1 NZ_CP079632:32970-33386 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T209565 NZ_CP104135:c1458796-1458509 [Sinorhizobium sp. C101]
ATGCCCTATAGCCTCGAATTCCTGCCGTCTGCTCGAAAGGAATGGGACAAGTTAGGCGCGACGATCCGGCAGCAGTTTGT
AAAGAAGCTGCGTGAGCGTTTGGAGCGTCCCCGCATTCCGAGCGCCGCGCTAAGCGGAATGCCCGACCACTACAAAATCA
AACTGAGACAGCTTGGCTACCGTCTCGTCTACAGGGTCGATGATGGCACTGTCATTGTGTTGGTCGTGGCAGTCGGGAAG
CGAGAACGGGGGGACGTCTACAACACAATGCGAGCACGCGGGCGTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT209565 WP_001261282.1 NZ_CP079632:32743-32973 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT209565 NZ_CP104135:c1459031-1458786 [Sinorhizobium sp. C101]
ATGCAGCGTGTAGAAGCCTCTGTAGCCGTGAGCGTTTCCGACTTGAAGAAGAGTCCCACGGCGGTAATGGGCGAGGCCCA
AGGCGAAGCGGTGGCCGTGCTGAACCACAATCGCGTCATGGCCTATCTGGTGCCTGCCGAGGTCTACGAATCCATGCTTG
AGCGGCTGGACGACCTCTATCTTGCCGAGCTCGTGAAAGAGCGAGCGGAGGAAAAGGGCGAGCCGGTCGATATCGATGCC
CTATAG

Similar Proteins


Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
No matching records found
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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
No matching records found

Structures


Toxin

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Source
ID
Structure
AlphaFold DBA0A0H3GYM2


Antitoxin

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Source
ID
Structure
AlphaFold DBA0A0H3GZG3

References