Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4818598..4819408 Replicon chromosome
Accession NZ_CP079629
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC18_CMC307MC

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KW554_RS23630 Protein ID WP_004178461.1
Coordinates 4818598..4819131 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KW554_RS23635 Protein ID WP_002887278.1
Coordinates 4819142..4819408 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW554_RS23625 (4817429) 4817429..4818550 + 1122 WP_002887282.1 cupin domain-containing protein -
KW554_RS23630 (4818598) 4818598..4819131 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KW554_RS23635 (4819142) 4819142..4819408 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KW554_RS23640 (4819511) 4819511..4820944 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
KW554_RS23645 (4820934) 4820934..4821617 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KW554_RS23650 (4821789) 4821789..4823174 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
KW554_RS23655 (4823192) 4823192..4823536 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T209559 WP_004178461.1 NZ_CP079629:c4819131-4818598 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T209559 NZ_CP104133:1245033-1245587 [Sinorhizobium sp. K101]
ATGAGGAAACACAGTCCGCTCGTATCGCGACCCGATCCGGAACCGACGGCGTCGGTCGAGCCGATAACGCTCTGGCGGGC
CTTCGTTCCACGCTGGGCACATATGCCGCTATCCGGCGCGGGTGCTGCTCGCTTCGGTGGCCGCTGGAACCCGATCGGCG
TGCCTGCGATCTATGGCGCGCGCGAGCTTTCCACCGCCTGGGCCGAATACAATCAGGGTTTCGTCCAGCATCCGGCGCTG
ATCGTGCAGCTCGAGTTGCTGGGGGCGAGGCTCGCGGACCTCACGGATGTCAGCCTTCTTGTGGAGCTTGGTGTGGATGA
GGCGATCCATCGCTGCGAATGGCGTGATGCGCTCGACAAGGGCGCGGTGCCGGAGACCCACAGGCTCCAGTCGGAGCTTC
TGGCGCGCGACTTCCATGGGGTGATCTATCCATCCTTCATGTCGCCCGGCGGCACCTGTGTTGCGCTCTGGCGTTGGAAC
GGTCCGCAAGAACCGCGTCTCGGCGTCATCGACCCCGATGGCCGGCTGCCGAAATCGCCCGCCTCATGGGTTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT209559 WP_002887278.1 NZ_CP079629:c4819408-4819142 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT209559 NZ_CP104133:1244655-1245026 [Sinorhizobium sp. K101]
ATGATTGCCCTCGATTTCCAGATCCCTGCAGCCCGTTTCGGGGACGATCATTCCCCGTTTCTCTCGGCCCGCCTGGTCGC
CGACCGATTGGGCGTCACCCTCGCCGAACTCGCCAAGCTGATCGGTGTCGCCCGCAATACGCTGACGGCGAAATCCGGCG
CCCGGAAGGTGGACAGCGCCTTGAGCAAGGTCGTCCGCATCCTGGCCATGGCTTCCGAAATGGCGGGCGACGAAGCCCGC
GCCGTCATCTGGTTCAAGCACCAGCCTATTCCCGGCTGGGCCGGCAAGACGGCATTCGACCTGGTCGGTGAAGGCAAGGC
CGACAAGGTGCTCGCCTATCTCGAATCGGTACGCGCCGGCGTCTACGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References