Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4222899..4223518 Replicon chromosome
Accession NZ_CP079629
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC18_CMC307MC

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KW554_RS20795 Protein ID WP_002892050.1
Coordinates 4223300..4223518 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KW554_RS20790 Protein ID WP_002892066.1
Coordinates 4222899..4223273 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW554_RS20780 (4218051) 4218051..4219244 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KW554_RS20785 (4219267) 4219267..4222413 + 3147 WP_218427596.1 multidrug efflux RND transporter permease subunit AcrB -
KW554_RS20790 (4222899) 4222899..4223273 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KW554_RS20795 (4223300) 4223300..4223518 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KW554_RS20800 (4223677) 4223677..4224243 + 567 WP_002892030.1 maltose O-acetyltransferase -
KW554_RS20805 (4224215) 4224215..4224355 - 141 WP_004147370.1 hypothetical protein -
KW554_RS20810 (4224376) 4224376..4224846 + 471 WP_002892026.1 YlaC family protein -
KW554_RS20815 (4224821) 4224821..4226272 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KW554_RS20820 (4226373) 4226373..4227071 + 699 WP_004178762.1 GNAT family protein -
KW554_RS20825 (4227068) 4227068..4227208 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KW554_RS20830 (4227208) 4227208..4227471 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T209558 WP_002892050.1 NZ_CP079629:4223300-4223518 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T209558 NZ_CP104133:124122-124529 [Sinorhizobium sp. K101]
ATGTATCTGGTTGACACCAACATCGTTTCGGAGGCGCGACGCGGCACGCCTCAGGCGCTGTCCTGGTTGCGTTCCGTCGA
TCCGCTCAGCATCCAACTGAGTGCACTGACCCTCGGCGAGATCATGCGCGGCATCGCACTGAAACAGAAGTCGGATCCGA
AGGCTGCCGCTCACCTCACCGAATGGCTGCGCAAGCTGCGCCACGACCATGGCGACCGCATCCTTCCGGTGACCGACCAG
ATCGCCGTCGAATGGGGCCGCATCGCCGCGATTCGGTCGCGTGGCGATATTGACGGGCTCCTCGCCGCGACCGCGATCGT
CCACGATCTGATCCTCGTCACGCGCAACGTCAGGGATTTTGAAGACACGGGCGCGTCAGTGATCAATCCTTGGGAGACAC
CGGCGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT209558 WP_002892066.1 NZ_CP079629:4222899-4223273 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT209558 NZ_CP104133:123868-124122 [Sinorhizobium sp. K101]
ATGGATTGGCAGCTGCAGGACGCGAAGAACCACTTCTCGAAAGTGGTGCAGAAGGCGCGCAACGAAGGGCCGCAGATCGT
CACGCTGCGTGGCGAGCGCACGGCCGTGGTGCTGTCCGCCCGCGACTATGACGCTCTGCGCGCCGGCCGGCCGACCCTGG
TCGACGATCTGCTTGGCGGTCCCCCTTGGGACGATGAGCTCGCCGAAGCCATCGAAGCGCGCGCCAAAACGCCGAGTCGC
GGCGTGGCCTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References