Detailed information of TA system TA020955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 536241..536887
• Replicon: chromosome
• Accession: NC_004757
• Organism: Nitrosomonas europaea ATCC 19718

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ALW85_RS02635
• Protein ID: WP_011111129.1
• Coordinates: 536477..536887 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A837WTB6
• Locus tag: ALW85_RS02630
• Protein ID: WP_041356451.1
• Coordinates: 536241..536480 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ALW85_RS13885	531738..531872	-	135	Protein_496	IS3 family transposase	-
ALW85_RS02580 (NE0490)	531968..532285	-	318	WP_011111121.1	HigA family addiction module antidote protein	-
ALW85_RS02585 (NE0491)	532298..532621	-	324	WP_011111122.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ALW85_RS02590 (NE0492)	532909..533976	-	1068	WP_011111123.1	Rpn family recombination-promoting nuclease/putative transposase	-
ALW85_RS02595 (NE0493)	533980..534294	-	315	WP_041356444.1	helix-turn-helix domain-containing protein	-
ALW85_RS02600 (NE0494)	534294..534614	-	321	WP_011111125.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ALW85_RS02620 (NE0496)	535662..535853	+	192	WP_011111127.1	hypothetical protein	-
ALW85_RS02630 (NE0497)	536241..536480	+	240	WP_041356451.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ALW85_RS02635 (NE0498)	536477..536887	+	411	WP_011111129.1	type II toxin-antitoxin system VapC family toxin	Toxin
ALW85_RS02640 (NE0499)	536923..537672	+	750	WP_011111130.1	glycosyltransferase	-
ALW85_RS02645 (NE0500)	537687..538634	+	948	WP_011111131.1	SDR family oxidoreductase	-
ALW85_RS02650 (NE0501)	538693..539253	+	561	WP_041357027.1	sugar transferase	-
ALW85_RS02655 (NE0502)	539262..541229	+	1968	WP_011111133.1	polysaccharide biosynthesis protein	-
ALW85_RS02660 (NE0503)	541277..541774	-	498	WP_011111134.1	DUF4124 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-127)

Antitoxin

• SpoVT-AbrB domain (10-52)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15118.58 Da
• Isoelectric Point: 5.1846

>T20955 WP_011111129.1 NC_004757:536477-536887 [Nitrosomonas europaea ATCC 19718]
MISPRYLLDTNILSDLVRYPQGVIARRIEEVGEAAVCTSIIVAAELRFGAARRNSLRLTRQVEAILAAIEVLPLDTPVDR
AYAQLRWVLEQSGQVIGPNDMLITAQAMASQCVLITANLDKFSRVGELQVENWLVR

Nucleotide

• Length: 411 bp

>T20955 NC_004757:536477-536887 [Nitrosomonas europaea ATCC 19718]
ATGATTTCACCGCGTTATTTACTGGATACGAATATTCTTTCTGATCTGGTTCGCTATCCACAAGGAGTTATAGCCCGACG
AATTGAGGAAGTTGGTGAAGCAGCGGTATGTACGTCGATTATCGTTGCCGCAGAATTGAGGTTTGGGGCTGCCAGGAGAA
ATTCCTTAAGGCTGACCAGGCAGGTGGAAGCCATTCTGGCGGCAATCGAGGTGTTGCCGCTTGATACGCCAGTGGACCGC
GCTTATGCGCAATTGCGCTGGGTGCTTGAGCAATCTGGACAAGTCATCGGGCCTAACGATATGCTGATTACGGCCCAGGC
TATGGCAAGTCAATGTGTGCTGATTACTGCCAATCTGGATAAATTTTCCCGGGTTGGGGAATTGCAGGTAGAAAACTGGT
TAGTGAGGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9127.58 Da
• Isoelectric Point: 4.6151

>AT20955 WP_041356451.1 NC_004757:536241-536480 [Nitrosomonas europaea ATCC 19718]
MLSERYVRLFRNGKNQAVRIPREFELNAQEVIMRREGNRLIIEPVPPKGLLVVLAELAPLEENFSDIDTRLAPLDDIDL

Nucleotide

• Length: 240 bp

>AT20955 NC_004757:536241-536480 [Nitrosomonas europaea ATCC 19718]
ATGTTATCTGAACGTTATGTCAGATTGTTTCGTAACGGCAAGAATCAGGCTGTGCGTATTCCTCGCGAATTCGAGCTGAA
TGCGCAGGAGGTAATCATGCGCCGGGAAGGTAATCGTTTGATTATTGAACCGGTACCCCCAAAAGGCTTGCTGGTAGTGT
TGGCAGAGTTGGCACCACTTGAGGAAAATTTTTCTGATATTGATACCAGATTGGCACCGTTGGATGACATTGATTTATGA

Structures

Antitoxin

• AlphaFold DB: A0A837WTB6