Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 58669..59405 Replicon plasmid pCMC307P_P1
Accession NZ_CP079625
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC17_CMC307PC

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KW552_RS27195 Protein ID WP_003026803.1
Coordinates 58923..59405 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KW552_RS27190 Protein ID WP_003026799.1
Coordinates 58669..58935 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW552_RS27145 (KW552_27130) 54731..55093 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
KW552_RS27150 (KW552_27135) 55143..55493 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KW552_RS27155 (KW552_27140) 55851..56120 + 270 WP_004152102.1 hypothetical protein -
KW552_RS27160 (KW552_27145) 56108..56683 + 576 WP_004152103.1 hypothetical protein -
KW552_RS27165 (KW552_27150) 56714..57208 + 495 WP_004152104.1 DNA-binding protein -
KW552_RS27170 (KW552_27155) 57252..57620 + 369 WP_004152105.1 hypothetical protein -
KW552_RS27175 (KW552_27160) 57654..57857 + 204 WP_004152106.1 HHA domain-containing protein -
KW552_RS27180 (KW552_27165) 57906..58163 + 258 WP_004152107.1 hypothetical protein -
KW552_RS27185 (KW552_27170) 58239..58493 + 255 WP_004152108.1 hypothetical protein -
KW552_RS27190 (KW552_27175) 58669..58935 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KW552_RS27195 (KW552_27180) 58923..59405 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KW552_RS27200 (KW552_27185) 59617..60963 + 1347 WP_020314316.1 ISNCY family transposase -
KW552_RS29865 61124..61255 + 132 WP_004218042.1 hypothetical protein -
KW552_RS27205 (KW552_27190) 61464..62629 - 1166 Protein_67 IS3 family transposase -
KW552_RS27210 (KW552_27195) 62806..63768 - 963 WP_004152113.1 zinc metalloprotease HtpX -
KW552_RS27215 (KW552_27200) 63755..64243 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 - 1..211805 211805


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T209543 WP_003026803.1 NZ_CP079625:58923-59405 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T209543 NZ_CP104128:459947-460330 [Sinorhizobium sp. M103]
GTGATTCTTGCCGACACTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTGTTGAGGACGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTCGGCAGTCTTCGGGAACGCAGTAGCGTGATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCACGAGGTCATGATGATGGTTGATCGCCACGCCATTTTCAGCATG
GGGATCGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTTCTCGATAAGCGAGTGGCTTTGTGGACGAGAGACAAGCG
TTTGCGGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCCGCCCATACACGGAACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT209543 WP_003026799.1 NZ_CP079625:58669-58935 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT209543 NZ_CP104128:459750-459950 [Sinorhizobium sp. M103]
ATGCGATCGACTATCAACCTCGACGACGCTCTCATGGAAACGGCCAAGTCCCTAACCGGCACGAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTCCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAGGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References