Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4833314..4834124 Replicon chromosome
Accession NZ_CP079624
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC17_CMC307PC

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KW552_RS23685 Protein ID WP_004178461.1
Coordinates 4833314..4833847 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KW552_RS23690 Protein ID WP_002887278.1
Coordinates 4833858..4834124 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW552_RS23680 (4832145) 4832145..4833266 + 1122 WP_002887282.1 cupin domain-containing protein -
KW552_RS23685 (4833314) 4833314..4833847 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KW552_RS23690 (4833858) 4833858..4834124 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KW552_RS23695 (4834227) 4834227..4835660 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
KW552_RS23700 (4835650) 4835650..4836333 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KW552_RS23705 (4836505) 4836505..4837890 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
KW552_RS23710 (4837908) 4837908..4838252 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T209539 WP_004178461.1 NZ_CP079624:c4833847-4833314 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T209539 NZ_CP104127:c1458788-1458501 [Sinorhizobium sp. M103]
ATGCCCTATAGCCTCGAATTCCTGCCGTCTGCTCGAAAGGAATGGGACAAGTTAGGCGCGACGATCCGGCAGCAGTTTGT
AAAGAAGCTGCGTGAGCGTTTGGAGCGTCCCCGCATTCCGAGCGCCGCGCTAAGCGGAATGCCCGACCACTACAAAATCA
AACTGAGACAGCTTGGCTACCGTCTCGTCTACAGGGTCGATGATGGCACTGTCATTGTGTTGGTCGTGGCAGTCGGGAAG
CGAGAACGGGGGGACGTCTACAACACAATGCGAGCACGCGGGCGTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT209539 WP_002887278.1 NZ_CP079624:c4834124-4833858 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT209539 NZ_CP104127:c1459023-1458778 [Sinorhizobium sp. M103]
ATGCAGCGTGTAGAAGCCTCTGTAGCCGTGAGCGTTTCCGACTTGAAGAAGAGTCCCACGGCGGTAATGGGCGAGGCCCA
AGGCGAAGCGGTGGCCGTGCTGAACCACAATCGCGTCATGGCCTATCTGGTGCCTGCCGAGGTCTACGAATCCATGCTTG
AGCGGCTGGACGACCTCTATCTTGCCGAGCTCGTGAAAGAGCGAGCGGAGGAAAAGGGCGAGCCGGTCGATATCGATGCC
CTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References