Detailed information of TA system TA020953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 519527..520161
• Replicon: chromosome
• Accession: NC_004757
• Organism: Nitrosomonas europaea ATCC 19718

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ALW85_RS02530
• Protein ID: WP_011111110.1
• Coordinates: 519760..520161 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q82X22
• Locus tag: ALW85_RS02525
• Protein ID: WP_011111109.1
• Coordinates: 519527..519760 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ALW85_RS02500 (NE0473)	514553..514918	+	366	WP_011111104.1	hypothetical protein	-
ALW85_RS02505 (NE0474)	515103..516257	+	1155	WP_011111105.1	ATP-binding protein	-
ALW85_RS02510 (NE0475)	516404..516625	+	222	WP_011111106.1	ribbon-helix-helix protein, CopG family	-
ALW85_RS02515 (NE0476)	516603..516857	+	255	WP_011111107.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ALW85_RS13875	517180..517371	+	192	WP_143023752.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ALW85_RS02520 (NE0477)	517316..519226	-	1911	WP_011111108.1	ATP-binding cassette domain-containing protein	-
ALW85_RS02525 (NE0478)	519527..519760	+	234	WP_011111109.1	antitoxin	Antitoxin
ALW85_RS02530 (NE0479)	519760..520161	+	402	WP_011111110.1	type II toxin-antitoxin system VapC family toxin	Toxin
ALW85_RS02535 (NE0480)	520320..520748	+	429	WP_011111111.1	hypothetical protein	-
ALW85_RS02540 (NE0481)	520813..521118	-	306	WP_011111112.1	HigA family addiction module antidote protein	-
ALW85_RS02545 (NE0482)	521128..521406	-	279	WP_041357019.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ALW85_RS02550 (NE0483)	521508..522863	+	1356	WP_011111114.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (9-56)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15343.62 Da
• Isoelectric Point: 7.4204

>T20953 WP_011111110.1 NC_004757:519760-520161 [Nitrosomonas europaea ATCC 19718]
MLKYMLDTNIAIYVIKRRPIEVLVTFNRYADMMCVSAVTEAELLHGAEKSRQREHNLRQVADFLSRLEVLSYTSKAAGHY
GDIRADLERKEKPIGVNDLHIAAHARSEGFILVSNNLREFERVDGLRLENWIT

Nucleotide

• Length: 402 bp

>T20953 NC_004757:519760-520161 [Nitrosomonas europaea ATCC 19718]
ATGCTCAAATATATGCTGGATACGAACATCGCCATCTACGTTATCAAACGTCGGCCAATAGAAGTGCTGGTTACCTTTAA
CCGCTATGCAGACATGATGTGCGTAAGCGCTGTTACCGAAGCCGAGTTACTGCACGGCGCAGAAAAAAGCCGGCAGCGTG
AGCACAATCTGCGACAGGTGGCTGATTTTTTATCTCGTCTGGAAGTGCTGTCGTATACCAGTAAAGCAGCCGGGCATTAT
GGTGATATTCGTGCTGATCTGGAGCGAAAAGAAAAGCCCATCGGCGTGAATGACTTGCACATCGCCGCTCATGCTCGCAG
TGAAGGTTTCATCCTGGTCAGTAATAACCTGCGTGAATTTGAACGAGTAGATGGTTTGCGTTTGGAAAATTGGATTACCT
GA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 9077.16 Da
• Isoelectric Point: 4.8096

>AT20953 WP_011111109.1 NC_004757:519527-519760 [Nitrosomonas europaea ATCC 19718]
MYTGTVFENNRTQAVRLPVDVRFADDVKKVWVRKLGKERILTPVDHTWDSFFLAEQGVSEDFLSERASQEQQEREVF

Nucleotide

• Length: 234 bp

>AT20953 NC_004757:519527-519760 [Nitrosomonas europaea ATCC 19718]
ATGTATACAGGTACTGTTTTTGAAAATAACCGTACTCAGGCTGTACGTTTGCCGGTAGATGTGCGCTTTGCCGATGATGT
GAAAAAGGTCTGGGTACGTAAACTGGGTAAAGAGCGAATCCTGACCCCGGTTGACCATACTTGGGACAGCTTTTTTCTGG
CGGAACAAGGTGTTTCAGAAGATTTTTTATCTGAACGTGCCAGTCAGGAGCAGCAGGAGCGCGAGGTGTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q82X22