Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5417277..5417902 Replicon chromosome
Accession NZ_CP079617
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC16_AI1547MC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KW551_RS26590 Protein ID WP_002882817.1
Coordinates 5417277..5417660 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KW551_RS26595 Protein ID WP_004150355.1
Coordinates 5417660..5417902 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW551_RS26575 (5414643) 5414643..5415545 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KW551_RS26580 (5415542) 5415542..5416177 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KW551_RS26585 (5416174) 5416174..5417103 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
KW551_RS26590 (5417277) 5417277..5417660 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KW551_RS26595 (5417660) 5417660..5417902 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KW551_RS26600 (5418107) 5418107..5419024 + 918 WP_004181612.1 alpha/beta hydrolase -
KW551_RS26605 (5419038) 5419038..5419979 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KW551_RS26610 (5420024) 5420024..5420461 - 438 WP_064162494.1 D-aminoacyl-tRNA deacylase -
KW551_RS26615 (5420458) 5420458..5421318 - 861 WP_004146232.1 virulence factor BrkB family protein -
KW551_RS26620 (5421312) 5421312..5421911 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209524 WP_002882817.1 NZ_CP079617:c5417660-5417277 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209524 NZ_CP104123:2115457-2115560 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209524 WP_004150355.1 NZ_CP079617:c5417902-5417660 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209524 NZ_CP104123:c2115598-2115327 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References