Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4200126..4200745 Replicon chromosome
Accession NZ_CP079617
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC16_AI1547MC

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KW551_RS20810 Protein ID WP_002892050.1
Coordinates 4200527..4200745 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KW551_RS20805 Protein ID WP_002892066.1
Coordinates 4200126..4200500 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW551_RS20795 (4195278) 4195278..4196471 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KW551_RS20800 (4196494) 4196494..4199640 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KW551_RS20805 (4200126) 4200126..4200500 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KW551_RS20810 (4200527) 4200527..4200745 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KW551_RS20815 (4200908) 4200908..4201474 + 567 WP_002892030.1 maltose O-acetyltransferase -
KW551_RS20820 (4201446) 4201446..4201586 - 141 WP_004147370.1 hypothetical protein -
KW551_RS20825 (4201607) 4201607..4202077 + 471 WP_002892026.1 YlaC family protein -
KW551_RS20830 (4202052) 4202052..4203503 - 1452 WP_064162152.1 PLP-dependent aminotransferase family protein -
KW551_RS20835 (4203604) 4203604..4204302 + 699 WP_004177238.1 GNAT family protein -
KW551_RS20840 (4204299) 4204299..4204439 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KW551_RS20845 (4204439) 4204439..4204702 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T209521 WP_002892050.1 NZ_CP079617:4200527-4200745 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T209521 NZ_CP104123:1430443-1430841 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT209521 WP_002892066.1 NZ_CP079617:4200126-4200500 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT209521 NZ_CP104123:1430216-1430443 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References