Detailed information of TA system TA209519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2775357..2776945
• Replicon: chromosome
• Accession: NZ_CP079617
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC16_AI1547MC

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KW551_RS13745
• Protein ID: WP_064162061.1
• Coordinates: 2775357..2776679 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: KW551_RS13750
• Protein ID: WP_002904790.1
• Coordinates: 2776679..2776945 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW551_RS13730 (2770413)	2770413..2772914	+	2502	WP_064162059.1	fimbria/pilus outer membrane usher protein	-
KW551_RS13735 (2772902)	2772902..2773906	+	1005	WP_064162060.1	fimbrial protein	-
KW551_RS13740 (2774048)	2774048..2775340	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
KW551_RS13745 (2775357)	2775357..2776679	-	1323	WP_064162061.1	type II toxin-antitoxin system HipA family toxin	Toxin
KW551_RS13750 (2776679)	2776679..2776945	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KW551_RS13755 (2777211)	2777211..2777537	-	327	WP_064162062.1	hydroxyisourate hydrolase	-
KW551_RS13760 (2777534)	2777534..2778034	-	501	WP_064162063.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KW551_RS13765 (2778012)	2778012..2779391	-	1380	WP_040245186.1	nucleobase:cation symporter-2 family protein	-
KW551_RS13770 (2779737)	2779737..2780891	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
KW551_RS13775 (2780872)	2780872..2781795	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49155.46 Da
• Isoelectric Point: 7.5357

>T209519 WP_064162061.1 NZ_CP079617:c2776679-2775357 [Klebsiella pneumoniae subsp. pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T209519 NZ_CP104123:1124580-1124915 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT209519 WP_002904790.1 NZ_CP079617:c2776945-2776679 [Klebsiella pneumoniae subsp. pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT209519 NZ_CP104123:1124332-1124580 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8