Detailed information of TA system TA209508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 53147..53672
• Replicon: plasmid pAI1547P_P3
• Accession: NZ_CP079609
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC15_AI1547PC

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: KW550_RS26975
• Protein ID: WP_001159868.1
• Coordinates: 53367..53672 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: KW550_RS26970
• Protein ID: WP_000813634.1
• Coordinates: 53147..53365 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW550_RS26940 (KW550_26915)	48540..48956	-	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	-
KW550_RS26945 (KW550_26920)	48953..49183	-	231	WP_001261278.1	type II toxin-antitoxin system VapB family antitoxin	-
KW550_RS26950 (KW550_26925)	49448..49948	+	501	WP_000528931.1	HEPN family nuclease	-
KW550_RS26955 (KW550_26930)	49961..50734	+	774	WP_000905949.1	hypothetical protein	-
KW550_RS26960 (KW550_26935)	50901..52034	+	1134	WP_000545987.1	DUF3800 domain-containing protein	-
KW550_RS26965 (KW550_26940)	52068..52556	-	489	WP_011254646.1	hypothetical protein	-
KW550_RS26970 (KW550_26945)	53147..53365	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KW550_RS26975 (KW550_26950)	53367..53672	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
KW550_RS26980 (KW550_26955)	53673..54479	+	807	WP_000016982.1	site-specific integrase	-
KW550_RS26985 (KW550_26960)	55253..56008	+	756	WP_000852146.1	replication initiation protein RepE	-
KW550_RS26990 (KW550_26965)	56596..57762	+	1167	WP_000772446.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / sitABCD	iucA / iucB / iucC / iucD / iutA	1..71307	71307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T209508 WP_001159868.1 NZ_CP079609:53367-53672 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T209508 NZ_CP104117:c4381685-4381335 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT209508 WP_000813634.1 NZ_CP079609:53147-53365 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT209508 NZ_CP104117:c4381930-4381679 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2