Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2980290..2980915 Replicon chromosome
Accession NZ_CP079606
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC15_AI1547PC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KW550_RS14435 Protein ID WP_002882817.1
Coordinates 2980290..2980673 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KW550_RS14440 Protein ID WP_004150355.1
Coordinates 2980673..2980915 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW550_RS14420 (2977656) 2977656..2978558 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KW550_RS14425 (2978555) 2978555..2979190 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KW550_RS14430 (2979187) 2979187..2980116 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
KW550_RS14435 (2980290) 2980290..2980673 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KW550_RS14440 (2980673) 2980673..2980915 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KW550_RS14445 (2981120) 2981120..2982037 + 918 WP_004181612.1 alpha/beta hydrolase -
KW550_RS14450 (2982051) 2982051..2982992 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KW550_RS14455 (2983037) 2983037..2983474 - 438 WP_064162494.1 D-aminoacyl-tRNA deacylase -
KW550_RS14460 (2983471) 2983471..2984331 - 861 WP_004146232.1 virulence factor BrkB family protein -
KW550_RS14465 (2984325) 2984325..2984924 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209495 WP_002882817.1 NZ_CP079606:c2980673-2980290 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209495 NZ_CP104117:1408259-1408657 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209495 WP_004150355.1 NZ_CP079606:c2980915-2980673 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209495 NZ_CP104117:1408032-1408259 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References