Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/DUF1778(antitoxin) |
| Location | 58669..59405 | Replicon | plasmid pCMC309M_P1 |
| Accession | NZ_CP079600 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain WRC10_CMC309MC | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | L7SZ15 |
| Locus tag | KWY78_RS25495 | Protein ID | WP_003026803.1 |
| Coordinates | 58923..59405 (+) | Length | 161 a.a. |
Antitoxin (Protein)
| Gene name | tacA | Uniprot ID | L7SZ29 |
| Locus tag | KWY78_RS25490 | Protein ID | WP_003026799.1 |
| Coordinates | 58669..58935 (+) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KWY78_RS25445 (KWY78_25430) | 54731..55093 | - | 363 | WP_004152100.1 | arsenite efflux transporter metallochaperone ArsD | - |
| KWY78_RS25450 (KWY78_25435) | 55143..55493 | - | 351 | WP_004152101.1 | As(III)-sensing metalloregulatory transcriptional repressor ArsR | - |
| KWY78_RS25455 (KWY78_25440) | 55851..56120 | + | 270 | WP_004152102.1 | hypothetical protein | - |
| KWY78_RS25460 (KWY78_25445) | 56108..56683 | + | 576 | WP_004152103.1 | hypothetical protein | - |
| KWY78_RS25465 (KWY78_25450) | 56714..57208 | + | 495 | WP_004152104.1 | DNA-binding protein | - |
| KWY78_RS25470 (KWY78_25455) | 57252..57620 | + | 369 | WP_004152105.1 | hypothetical protein | - |
| KWY78_RS25475 (KWY78_25460) | 57654..57857 | + | 204 | WP_004152106.1 | HHA domain-containing protein | - |
| KWY78_RS25480 (KWY78_25465) | 57906..58163 | + | 258 | WP_004152107.1 | hypothetical protein | - |
| KWY78_RS25485 (KWY78_25470) | 58239..58493 | + | 255 | WP_004152108.1 | hypothetical protein | - |
| KWY78_RS25490 (KWY78_25475) | 58669..58935 | + | 267 | WP_003026799.1 | DUF1778 domain-containing protein | Antitoxin |
| KWY78_RS25495 (KWY78_25480) | 58923..59405 | + | 483 | WP_003026803.1 | GNAT family N-acetyltransferase | Toxin |
| KWY78_RS25500 (KWY78_25485) | 59617..60963 | + | 1347 | WP_020314316.1 | ISNCY family transposase | - |
| KWY78_RS27235 | 61124..61255 | + | 132 | WP_256357619.1 | hypothetical protein | - |
| KWY78_RS25505 (KWY78_25490) | 61464..62629 | - | 1166 | Protein_67 | IS3 family transposase | - |
| KWY78_RS25510 (KWY78_25495) | 62806..63768 | - | 963 | WP_004152113.1 | zinc metalloprotease HtpX | - |
| KWY78_RS25515 (KWY78_25500) | 63755..64243 | - | 489 | WP_004152114.1 | phosphate-starvation-inducible PsiE family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | catA1 | - | 1..213013 | 213013 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 161 a.a. Molecular weight: 17353.97 Da Isoelectric Point: 9.5822
>T209489 WP_003026803.1 NZ_CP079600:58923-59405 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP
Download Length: 483 bp
>T209489 NZ_CP104115:74603-74953 [Escherichia coli]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
Antitoxin
Download Length: 89 a.a. Molecular weight: 10147.59 Da Isoelectric Point: 4.5256
>AT209489 WP_003026799.1 NZ_CP079600:58669-58935 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV
Download Length: 267 bp
>AT209489 NZ_CP104115:74958-75260 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0J2GNW6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1Q8YL66 |