Detailed information of TA system TA209481

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 2805480..2806137
• Replicon: chromosome
• Accession: NZ_CP079599
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC10_CMC309MC

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: KWY78_RS13605
• Protein ID: WP_004181233.1
• Coordinates: 2805480..2805890 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: KWY78_RS13610
• Protein ID: WP_002916312.1
• Coordinates: 2805871..2806137 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWY78_RS13585 (2801480)	2801480..2803213	-	1734	WP_004149758.1	single-stranded-DNA-specific exonuclease RecJ	-
KWY78_RS13590 (2803219)	2803219..2803932	-	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
KWY78_RS13595 (2803955)	2803955..2804851	-	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
KWY78_RS13600 (2804952)	2804952..2805473	+	522	WP_004144730.1	flavodoxin FldB	-
KWY78_RS13605 (2805480)	2805480..2805890	-	411	WP_004181233.1	protein YgfX	Toxin
KWY78_RS13610 (2805871)	2805871..2806137	-	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
KWY78_RS13615 (2806383)	2806383..2807366	+	984	WP_002916313.1	tRNA-modifying protein YgfZ	-
KWY78_RS13620 (2807517)	2807517..2808176	-	660	WP_002916317.1	hemolysin III family protein	-
KWY78_RS13625 (2808340)	2808340..2808651	-	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
KWY78_RS13630 (2808701)	2808701..2809429	+	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
KWY78_RS13635 (2809548)	2809548..2810981	+	1434	WP_004181234.1	6-phospho-beta-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16121.92 Da
• Isoelectric Point: 11.1565

>T209481 WP_004181233.1 NZ_CP079599:c2805890-2805480 [Klebsiella pneumoniae subsp. pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAEEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T209481 NZ_CP104114:c2865197-2864943 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT209481 WP_002916312.1 NZ_CP079599:c2806137-2805871 [Klebsiella pneumoniae subsp. pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT209481 NZ_CP104114:c2865445-2865194 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GY41