Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2966420..2967074 Replicon chromosome
Accession NC_004741
Organism Shigella flexneri 2a str. 2457T

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag S_RS16085 Protein ID WP_000244767.1
Coordinates 2966420..2966827 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag S_RS16090 Protein ID WP_000354046.1
Coordinates 2966808..2967074 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
S_RS16065 2962377..2964110 - 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -
S_RS16070 2964116..2964826 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
S_RS16075 2964851..2965747 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
S_RS16080 2965859..2966380 + 522 WP_001055867.1 flavodoxin FldB -
S_RS16085 2966420..2966827 - 408 WP_000244767.1 protein YgfX Toxin
S_RS16090 2966808..2967074 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
S_RS16095 2967317..2968297 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
S_RS16100 2968493..2969152 - 660 WP_000250274.1 hemolysin III family protein -
S_RS16105 2969316..2969626 - 311 Protein_2933 N(4)-acetylcytidine aminohydrolase -
S_RS16110 2969671..2971104 + 1434 WP_005051722.1 6-phospho-beta-glucosidase BglA -
S_RS16115 2971161..2971904 - 744 WP_000951957.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T20948 WP_000244767.1 NC_004741:c2966827-2966420 [Shigella flexneri 2a str. 2457T]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T20948 NC_004741:c2966827-2966420 [Shigella flexneri 2a str. 2457T]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT20948 WP_000354046.1 NC_004741:c2967074-2966808 [Shigella flexneri 2a str. 2457T]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT20948 NC_004741:c2967074-2966808 [Shigella flexneri 2a str. 2457T]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References